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BMRB: 5722


5722
1OQP
Chemical Shift Assignmens of Ca2+/C-terminal Domain of Caltractin (1H, 13C, and 15N) in Complex with the Cdc31p-Binding Domain from Kar1p (1H)
Authors
Hu, H., Chazin, W.J.
Assembly
complex between Ca2+/C-terminal domain of caltractin and 19-resiude peptide from Kar1p
Entity
1. C-terminal domain of caltractin (polymer, Thiol state: not present), 77 monomers, 8965.738 Da Detail

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF


2. 19-residue cdc31p-binding domain from Kar1p (polymer, Thiol state: not present), 19 monomers, 2491.931 Da Detail

KKRELIESKW HRLLFHDKK


3. CA (non-polymer), 40.078 Da
Total weight
11497.747 Da
Max. entity weight
8965.738 Da
Source organism
Chlamydomonas reinhardtii , Saccharomyces cerevisiae
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 82.3 %, Completeness: 74.0 %, Completeness (bb): 79.3 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All74.0 % (863 of 1167)74.8 % (463 of 619)71.7 % (320 of 446)78.4 % (80 of 102)
Backbone79.3 % (457 of 576)80.6 % (158 of 196)79.2 % (225 of 284)77.1 % (74 of 96)
Sidechain69.8 % (477 of 683)71.9 % (304 of 423)66.1 % (168 of 254)83.3 % (5 of 6)
Aromatic17.1 % (12 of 70)34.3 % (12 of 35) 0.0 % (0 of 34) 0.0 % (0 of 1)
Methyl84.8 % (78 of 92)87.0 % (40 of 46)82.6 % (38 of 46)

1. C-terminal domain of caltractin

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF

2. 19-residue cdc31p-binding domain from Kar1p

KKRELIESKW HRLLFHDKK

Show sample condition
Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1C-terminal domain of caltractin[U-13C; U-15N]1.0 mM
219-residue cdc31p-binding domain from Kar1p1.2 mM

Chem. Shift Complete2
Sequence coverage: 35.4 %, Completeness: 48.4 %, Completeness (bb): 48.5 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All48.4 % (1129 of 2334)53.6 % (664 of 1238)41.4 % (369 of 892)47.1 % (96 of 204)
Backbone48.5 % (559 of 1152)54.6 % (214 of 392)45.1 % (256 of 568)46.4 % (89 of 192)
Sidechain47.9 % (654 of 1366)53.1 % (449 of 846)39.2 % (199 of 508)50.0 % (6 of 12)
Aromatic16.4 % (23 of 140)32.9 % (23 of 70) 0.0 % (0 of 68) 0.0 % (0 of 2)
Methyl51.1 % (94 of 184)56.5 % (52 of 92)45.7 % (42 of 92)

1. C-terminal domain of caltractin

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF

2. 19-residue cdc31p-binding domain from Kar1p

KKRELIESKW HRLLFHDKK

Show sample condition
Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1C-terminal domain of caltractin[U-13C; U-15N]1.0 mM
219-residue cdc31p-binding domain from Kar1p1.2 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1OQP, Strand ID: A, B Detail

Release date
2003-06-24
Citation
Unique features in the C-terminal domain provide caltractin with target specificity
Hu, H., Chazin, W.J.
J. Mol. Biol. (2003), 330, 473-484, PubMed 12842464 , DOI: ,
Related entities 1. C-terminal domain of caltractin, : 1 : 1 : 352 entities Detail
More details for 354 related entities
Related entities 2. 19-residue cdc31p-binding domain from Kar1p, : 1 : 2 entities Detail
More details for 3 related entities
Interaction partners 2. 19-residue cdc31p-binding domain from Kar1p, : 6 interactors Detail
More details for 6 interactors identified by 4 citations
Experiments performed 1 experiments Detail
nullKeywords caltractin, centrin, kar1p, caltractin/kar1p complex, calcium signaling