BMRB: 5722

Chemical Shift Assignmens of Ca2+/C-terminal Domain of Caltractin (1H, 13C, and 15N) in Complex with the Cdc31p-Binding Domain from Kar1p (1H)

Authors

Hu, H., Chazin, W.J.

Assembly

complex between Ca2+/C-terminal domain of caltractin and 19-resiude peptide from Kar1p

Entity

1. C-terminal domain of caltractin (polymer, Thiol state: not present), 77 monomers, 8965.738 Da Detail

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF

2. 19-residue cdc31p-binding domain from Kar1p (polymer, Thiol state: not present), 19 monomers, 2491.931 Da Detail

KKRELIESKW HRLLFHDKK

3. CA (non-polymer), 40.078 Da

Total weight

11497.747 Da

Max. entity weight

8965.738 Da

Source organism

Chlamydomonas reinhardtii , Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 82.3 %, Completeness: 74.0 %, Completeness (bb): 79.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.0 % (863 of 1167)	74.8 % (463 of 619)	71.7 % (320 of 446)	78.4 % (80 of 102)
Backbone	79.3 % (457 of 576)	80.6 % (158 of 196)	79.2 % (225 of 284)	77.1 % (74 of 96)
Sidechain	69.8 % (477 of 683)	71.9 % (304 of 423)	66.1 % (168 of 254)	83.3 % (5 of 6)
Aromatic	17.1 % (12 of 70)	34.3 % (12 of 35)	0.0 % (0 of 34)	0.0 % (0 of 1)
Methyl	84.8 % (78 of 92)	87.0 % (40 of 46)	82.6 % (38 of 46)

1. C-terminal domain of caltractin

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF

2. 19-residue cdc31p-binding domain from Kar1p

KKRELIESKW HRLLFHDKK

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of caltractin	[U-13C; U-15N]		1.0 mM
2	19-residue cdc31p-binding domain from Kar1p			1.2 mM

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Chem. Shift Complete2

Sequence coverage: 35.4 %, Completeness: 48.4 %, Completeness (bb): 48.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	48.4 % (1129 of 2334)	53.6 % (664 of 1238)	41.4 % (369 of 892)	47.1 % (96 of 204)
Backbone	48.5 % (559 of 1152)	54.6 % (214 of 392)	45.1 % (256 of 568)	46.4 % (89 of 192)
Sidechain	47.9 % (654 of 1366)	53.1 % (449 of 846)	39.2 % (199 of 508)	50.0 % (6 of 12)
Aromatic	16.4 % (23 of 140)	32.9 % (23 of 70)	0.0 % (0 of 68)	0.0 % (0 of 2)
Methyl	51.1 % (94 of 184)	56.5 % (52 of 92)	45.7 % (42 of 92)

1. C-terminal domain of caltractin

GSGERDSREE ILKAFRLFDD DNSGTITIKD LRRVAKELGE NLTEEELQEM IAEADRNDDN EIDEDEFIRI MKKTSLF

2. 19-residue cdc31p-binding domain from Kar1p

KKRELIESKW HRLLFHDKK

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of caltractin	[U-13C; U-15N]		1.0 mM
2	19-residue cdc31p-binding domain from Kar1p			1.2 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1OQP, Strand ID: A, B Detail

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Release date

2003-06-24

Citation

Unique features in the C-terminal domain provide caltractin with target specificity
Hu, H., Chazin, W.J.
J. Mol. Biol. (2003), 330, 473-484, PubMed 12842464 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. C-terminal domain of caltractin, : 1 : 1 : 352 entities Detail

Related entities 2. 19-residue cdc31p-binding domain from Kar1p, : 1 : 2 entities Detail

Interaction partners 2. 19-residue cdc31p-binding domain from Kar1p, : 6 interactors Detail

Experiments performed 1 experiments Detail