BMRBj - BMREntryMaster

Found 1 Document (2.054 seconds)

BMRB: 5742

1ON4

Solution structure of soluble domain of Sco1 from Bacillus Subtilis

Authors

Balatri, E., Banci, L., Bertini, I., Cantini, F., Ciofi-Baffoni, S.

Assembly

COX assembly protein

Entity

1. COX assembly protein (polymer, Thiol state: all free), 174 monomers, 19834.14 Da Detail

HMLEIKDPLN YEVEPFTFQN QDGKNVSLES LKGEVWLADF IFTNCETICP PMTAHMTDLQ KKLKAENIDV RIISFSVDPE NDKPKQLKKF AANYPLSFDN WDFLTGYSQS EIEEFALKSF KAIVKKPEGE DQVIHQSSFY LVGPDGKVLK DYNGVENTPY DDIISDVKSA STLK

Formula weight

19834.14 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled to simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 73.6 %, Completeness (bb): 74.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.6 % (1540 of 2093)	85.2 % (930 of 1092)	53.5 % (438 of 818)	94.0 % (172 of 183)
Backbone	74.6 % (764 of 1024)	94.5 % (326 of 345)	55.3 % (285 of 515)	93.3 % (153 of 164)
Sidechain	72.9 % (901 of 1236)	80.2 % (599 of 747)	60.2 % (283 of 470)	100.0 % (19 of 19)
Aromatic	30.4 % (59 of 194)	58.8 % (57 of 97)	0.0 % (0 of 95)	100.0 % (2 of 2)
Methyl	90.3 % (159 of 176)	92.0 % (81 of 88)	88.6 % (78 of 88)

1. Sco1

HMLEIKDPLN YEVEPFTFQN QDGKNVSLES LKGEVWLADF IFTNCETICP PMTAHMTDLQ KKLKAENIDV RIISFSVDPE NDKPKQLKKF AANYPLSFDN WDFLTGYSQS EIEEFALKSF KAIVKKPEGE DQVIHQSSFY LVGPDGKVLK DYNGVENTPY DDIISDVKSA STLK

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.62 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.62 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1ON4, Strand ID: A Detail

Release date

2003-12-16

Citation

Solution structure of Sco1: a thioredoxin-like protein Involved in cytochrome c oxidase assembly
Balatri, E., Banci, L., Bertini, I., Cantini, F., Ciofi-Baffoni, S.
Structure (2003), 11, 1431-1443, PubMed 14604533 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P54178 released on 1996-10-01
Title SCO1_BACSU Entity SCO1 protein homolog

Related entities 1. COX assembly protein, : 1 : 64 entities Detail

Experiments performed 9 experiments Detail

1 CBCANH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

2 CBCA(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

3 HNCO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

4 HN(CA)CO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

5 (H)CCH-TOCSY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

6 2D NOESY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

7 3D 15N-separated NOESY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

8 3D 13C-separated NOESY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

9 HNHA

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker AVANCE (600 MHz)

#3 Model Bruker AVANCE (700 MHz)

#4 Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sco1	[U-15N; U-13C]	1.5 mM
2	potassium phosphate		100 mM
3	DTT		2 mM
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 7 (±0.2)

#	Name	Concentration
5	Sco1	1.5 mM
6	potassium phosphate	100 mM
7	DTT	2 mM
8	D2O	90 %

nullKeywords COX assembly protein, copper protein, NMR structure, Sco1 from B. subtilis, thioredoxin-like fold, YPMQ

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Found 1 Document (2.054 seconds)

BMRB: 5742

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. COX assembly protein, : 1 : 64 entities Detail

Experiments performed 9 experiments Detail

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