BMRB: 5748

Assignment of 1H, 13C, and 15N Resonances of the ARID Domain of P270

Authors

Kim, S.

Assembly

p270 or Human SWI1

Entity

1. p270 or Human SWI1 (polymer, Thiol state: not reported), 126 monomers, 14391.23 Da Detail

SSTTTNEKIT KLYELGGEPE RKMWVDRYLA FTEEKAMGMT NLPAVGRKPL DLYRLYVSVK EIGGLTQVNK NKKWRELATN LNVGTSSSAA SSLKKQYIQC LYAFECKIER GEDPPPDIFA HHHHHH

Formula weight

14391.23 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 80.0 %, Completeness (bb): 79.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.0 % (1197 of 1497)	88.5 % (693 of 783)	67.1 % (391 of 583)	86.3 % (113 of 131)
Backbone	79.3 % (590 of 744)	92.9 % (236 of 254)	65.7 % (243 of 370)	92.5 % (111 of 120)
Sidechain	82.2 % (716 of 871)	86.4 % (457 of 529)	77.6 % (257 of 331)	18.2 % (2 of 11)
Aromatic	32.5 % (41 of 126)	65.1 % (41 of 63)	0.0 % (0 of 61)	0.0 % (0 of 2)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. SWI1

SSTTTNEKIT KLYELGGEPE RKMWVDRYLA FTEEKAMGMT NLPAVGRKPL DLYRLYVSVK EIGGLTQVNK NKKWRELATN LNVGTSSSAA SSLKKQYIQC LYAFECKIER GEDPPPDIFA HHHHHH

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Sample

Temperature 293 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SWI1			0.5 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: 0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 8 models in PDB: 1RYU, Strand ID: A Detail

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Release date

2003-09-04

Citation

Assignment of 1H, 13C and 15N resonances of the ARID domain of P270
Kim, S., Zhu, L., Yu, B., Chen, Y.
J. Biomol. NMR (2003), 27, 277-278, PubMed 12975585 , DOI: , Abstract

Related entities 1. p270 or Human SWI1, : 1 : 7 : 46 entities Detail

Interaction partners 1. p270 or Human SWI1, : 31 interactors Detail

Experiments performed 8 experiments Detail