BMRBj - BMREntryMaster

Found 1 Document (1.703 seconds)

BMRB: 5753

1NG7

Towards an Understanding of the Poliovirus Replication Complex: The Solution Structure of the Soluble Domain of the Poliovirus 3A Protein

Authors

Strauss, D.M., Glustrom, L.W., Wuttke, D.S.

Assembly

Genome polyprotein [Core protein P3A]

Entity

1. Genome polyprotein [Core protein P3A] (polymer, Thiol state: not reported), 60 monomers, 6903.780 × 2 Da Detail

MGPLQYKDLK IDIKTSPPPE CINDLLQAVD SQEVRDYCEK KGWIVNITSQ VQTERNINRA

Total weight

13807.56 Da

Max. entity weight

6903.78 Da

Source organism

Enterovirus C

Exptl. method

NMR

Refine. method

distance geometry/simmulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.4 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.4 % (620 of 718)	82.9 % (315 of 380)	89.7 % (244 of 272)	92.4 % (61 of 66)
Backbone	95.2 % (335 of 352)	94.9 % (112 of 118)	94.9 % (169 of 178)	96.4 % (54 of 56)
Sidechain	80.2 % (340 of 424)	77.5 % (203 of 262)	85.5 % (130 of 152)	70.0 % (7 of 10)
Aromatic	46.4 % (13 of 28)	71.4 % (10 of 14)	15.4 % (2 of 13)	100.0 % (1 of 1)
Methyl	90.9 % (60 of 66)	90.9 % (30 of 33)	90.9 % (30 of 33)

1. Poliovirus 3A

MGPLQYKDLK IDIKTSPPPE CINDLLQAVD SQEVRDYCEK KGWIVNITSQ VQTERNINRA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	Poliovirus 3A	[U-95% 13C; U-99% 15N]	1.5 mM
2	potassium phosphate		20 mM
3	NaCl		50 mM
4	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061
³¹P	DSS	methyl protons	null	indirect	0.4048086

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1NG7, Strand ID: A, B Detail

Release date

2003-08-07

Citation

Towards an understanding of the poliovirus replication complex: the solution structure of the soluble domain of the poliovirus 3A protein
Strauss, D.M., Glustrom, L.W., Wuttke, D.S.
J. Mol. Biol. (2003), 330, 225-234, PubMed 12823963 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P03300 released on 1986-07-21
Title POLG_POL1M Entity Capsid protein VP1

Related entities 1. Genome polyprotein [Core protein P3A], : 1 : 4 : 37 entities Detail

Experiments performed 4 experiments Detail

#	Name	Isotope labeling	Concentration
5	Poliovirus 3A	[U-95% 13C;U-99% 15N]	2.0 mM
6	potassium phosphate		20 mM
7	NaCl		50 mM
8	H2O		95 %
9	D2O		5 %

#	Name	Isotope labeling	Concentration
5	Poliovirus 3A	[U-95% 13C;U-99% 15N]	2.0 mM
6	potassium phosphate		20 mM
7	NaCl		50 mM
8	H2O		95 %
9	D2O		5 %

#	Name	Isotope labeling	Concentration
5	Poliovirus 3A	[U-95% 13C;U-99% 15N]	2.0 mM
6	potassium phosphate		20 mM
7	NaCl		50 mM
8	H2O		95 %
9	D2O		5 %

#	Name	Isotope labeling	Concentration
5	Poliovirus 3A	[U-95% 13C;U-99% 15N]	2.0 mM
6	potassium phosphate		20 mM
7	NaCl		50 mM
8	H2O		95 %
9	D2O		5 %

nullKeywords Poliovirus 3A protein, picornavirus, symmetric dimer structure, unfolded domain

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Found 1 Document (1.703 seconds)

BMRB: 5753

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Genome polyprotein [Core protein P3A], : 1 : 4 : 37 entities Detail

Experiments performed 4 experiments Detail

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