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Found 1 Document (1.251 seconds)

BMRB: 5758

1OVQ

1H, 13C and 15N chemical shift assignments for YqgF

Authors

Liu, D., Repaka, P., Taremi, S.S., Wyss, D.F.

Assembly

YqgF

Entity

1. YqgF (polymer, Thiol state: not present), 147 monomers, 16061.07 Da Detail

RGSMADIGSM SGTLLAFDFG TKSIGVAVGQ RITGTARPLP AIKAQDGTPD WNIIERLLKE WQPDEIIVGL PLNMDGTEQP LTARARKFAN RIHGRFGVEV KLHDERLSTV EARSGLFEQG GYRALNKGKV DSASAVIILE SYFEQGY

Formula weight

16061.07 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.0 %, Completeness: 73.8 %, Completeness (bb): 80.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.8 % (1239 of 1678)	72.8 % (635 of 872)	73.8 % (482 of 653)	79.7 % (122 of 153)
Backbone	80.8 % (703 of 870)	79.0 % (241 of 305)	82.3 % (349 of 424)	80.1 % (113 of 141)
Sidechain	69.1 % (648 of 938)	69.5 % (394 of 567)	68.2 % (245 of 359)	75.0 % (9 of 12)
Aromatic	56.9 % (66 of 116)	74.1 % (43 of 58)	37.5 % (21 of 56)	100.0 % (2 of 2)
Methyl	90.6 % (154 of 170)	90.6 % (77 of 85)	90.6 % (77 of 85)

1. YqgF

RGSMADIGSM SGTLLAFDFG TKSIGVAVGQ RITGTARPLP AIKAQDGTPD WNIIERLLKE WQPDEIIVGL PLNMDGTEQP LTARARKFAN RIHGRFGVEV KLHDERLSTV EARSGLFEQG GYRALNKGKV DSASAVIILE SYFEQGY

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1OVQ, Strand ID: A Detail

Release date

2003-10-15

Citation

Solution structure of the hypothetical protein YqgF from Escherichia coli reveals an RNAse H fold
Liu, D., Wang, Y., Wyss, D.F.
J. Biomol. NMR (2003), 27, 389-392, PubMed 14512736 , DOI 10.2210/pdb1ovq/pdb , Abstract

Related entities 1. YqgF, : 1 : 4 : 105 entities Detail

Interaction partners 1. YqgF, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 1H-15N NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

2 1H-15N TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

3 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

4 HN(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

5 HN(CO)

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

6 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

7 CBCACONNH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

8 13C/15N edited NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

9 HCCH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	YqgF	[U-15N]		0.3 ~ 1.0 mM
2	KiPO4			75 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	YqgF	[U-13C; U-15N]		0.5 ~ 1.0 mM
4	KiPO4			75 mM

nullKeywords YqgF, structural genomics, NMR assignments, antibiotics resistance, anti-microbial

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Found 1 Document (1.251 seconds)

BMRB: 5758

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YqgF, : 1 : 4 : 105 entities Detail

Interaction partners 1. YqgF, : 2 interactors Detail

Experiments performed 9 experiments Detail

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