BMRB: 5765

NMR studies of the fifth transmembrane segment of sarcoplasmic reticulum Ca2+-ATPase reveals a hinge close to the Ca2+ ligating residues

Authors

Nielsen, G., Malmendal, A., Meissner, A., Moller, J.V., Nielsen, N.

Assembly

fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase

Entity

1. fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase (polymer, Thiol state: not present), 28 monomers, 3065.560 Da Detail

KQFIRYLISS NVGEVVSIFL TAALGLPE

Formula weight

3065.56 Da

Source organism

Oryctolagus cuniculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.9 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.9 % (163 of 170)	95.9 % (163 of 170)
Backbone	100.0 % (57 of 57)	100.0 % (57 of 57)
Sidechain	93.8 % (106 of 113)	93.8 % (106 of 113)
Aromatic	92.9 % (13 of 14)	92.9 % (13 of 14)
Methyl	91.3 % (21 of 23)	91.3 % (21 of 23)

1. fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase

KQFIRYLISS NVGEVVSIFL TAALGLPE

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Sample

Temperature 313 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase			2.4 mM
2	SDS-d25			400 mM
3	NaCl			100 mM
4	sodium phosphate			20 mM
5	NaN3			1 mM
6	D2O			10 %

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Release date

2003-08-07

Citation

NMR studies of the fifth transmembrane segment of sarcoplasmic reticulum Ca2+-ATPase reveals a hinge close to the Ca2+-ligating residues
Nielsen, G., Malmendal, A., Meissner, A., Moller, J.V., Nielsen, N.
FEBS Lett. (2003), 544, 50-56, PubMed 12782289 , DOI: , Abstract

Related entities 1. fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase, : 1 : 5 : 91 : 35 entities Detail

Interaction partners 1. fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase, : 14 interactors Detail

Experiments performed 1 experiments Detail