NMR studies of the fifth transmembrane segment of sarcoplasmic reticulum Ca2+-ATPase reveals a hinge close to the Ca2+ ligating residues
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 95.9 % (163 of 170) | 95.9 % (163 of 170) |
Backbone | 100.0 % (57 of 57) | 100.0 % (57 of 57) |
Sidechain | 93.8 % (106 of 113) | 93.8 % (106 of 113) |
Aromatic | 92.9 % (13 of 14) | 92.9 % (13 of 14) |
Methyl | 91.3 % (21 of 23) | 91.3 % (21 of 23) |
1. fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase
KQFIRYLISS NVGEVVSIFL TAALGLPETemperature 313 (±1) K, pH 7.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | fifth transmembrane segment (residue L758 to E785) of sarcoplasmic reticulum Ca2+-ATPase | 2.4 mM | ||
2 | SDS-d25 | 400 mM | ||
3 | NaCl | 100 mM | ||
4 | sodium phosphate | 20 mM | ||
5 | NaN3 | 1 mM | ||
6 | D2O | 10 % |