BMRB: 5766

Solution Structure of the Lyase Domain of Human DNA Polymerase Lambda

Authors

DeRose, E.F., Kirby, T.W., Mueller, G.A., Bebenek, K., Garcia-Diaz, M., Blanco, L., Kunkel, T.A., London, R.E.

Assembly

DNA polymerase lambda (E.C.2.7.7.7)

Entity

1. DNA polymerase lambda (E.C.2.7.7.7) (polymer, Thiol state: all free), 87 monomers, 9697.097 Da Detail

MAQPSSQKAT NHNLHITEKL EVLAKAYSVQ GDKWRALGYA KAINALKSFH KPVTSYQEAC SIPGIGKRMA EKIIEILESG HLRKLDH

Formula weight

9697.097 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 74.6 %, Completeness (bb): 86.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.6 % (767 of 1028)	68.0 % (366 of 538)	81.9 % (326 of 398)	81.5 % (75 of 92)
Backbone	86.6 % (447 of 516)	85.8 % (151 of 176)	86.7 % (222 of 256)	88.1 % (74 of 84)
Sidechain	66.2 % (393 of 594)	59.4 % (215 of 362)	79.0 % (177 of 224)	12.5 % (1 of 8)
Aromatic	27.3 % (18 of 66)	27.3 % (9 of 33)	25.0 % (8 of 32)	100.0 % (1 of 1)
Methyl	87.8 % (86 of 98)	85.7 % (42 of 49)	89.8 % (44 of 49)

1. 50S Ribosomal Protein L18

MAQPSSQKAT NHNLHITEKL EVLAKAYSVQ GDKWRALGYA KAINALKSFH KPVTSYQEAC SIPGIGKRMA EKIIEILESG HLRKLDH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	50S Ribosomal Protein L18	[U-15N; U-13C]		2 mM
2	Tris-d11			5 mM
3	NaCl			100 mM
4	DTT			1 mM
5	NaN3			5 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.35 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl	0.0 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.35 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl	0.0 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl	0.0 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: -0.6 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl	0.0 ppm	internal	direct	1.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 8 models in PDB: 1NZP, Strand ID: A Detail

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Release date

2003-09-07

Citation

Solution structure of the lyase domain of human DNA polymerase lambda
DeRose, E.F., Kirby, T.W., Mueller, G.A., Bebenek, K., Garcia-Diaz, M., Blanco, L., Kunkel, T.A., London, R.E.
Biochemistry (2003), 42, 9564-9574, PubMed 12911298 , DOI 10.1021/bi034298s , Abstract

Related entities 1. DNA polymerase lambda (E.C.2.7.7.7), : 1 : 20 : 38 : 73 entities Detail

Interaction partners 1. DNA polymerase lambda (E.C.2.7.7.7), : 14 interactors Detail

Experiments performed 2 experiments Detail