BMRB: 5768

Solution structure of Copper-CopAS46V from Bacillus subtilis

Authors

Banci, L., Bertini, I., Ciofi-Baffoni, S., Gonnelli, L., Su, X.C.

Assembly

Potential copper-transporting ATPase

Entity

1. CPx-type ATPase CopA (polymer, Thiol state: all other bound), 76 monomers, 8165.353 Da Detail

MLSEQKEIAM QVSGMTCAAC AARIEKGLKR MPGVTDANVN LATETVNVIY DPAETGTAAI QEKIEKLGYH VVIEGR

2. CU1 (non-polymer), 63.546 Da

Total weight

8228.899 Da

Max. entity weight

8165.353 Da

Entity Connection

na 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS17:SG	2:CU11:CU
2	na	sing	1:CYS20:SG	2:CU11:CU

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

distance geometry,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 88.3 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	88.3 % (452 of 512)	86.8 % (375 of 432)	96.2 % (77 of 80)
Backbone	95.7 % (220 of 230)	95.5 % (149 of 156)	95.9 % (71 of 74)
Sidechain	82.3 % (232 of 282)	81.9 % (226 of 276)	100.0 % (6 of 6)
Aromatic	40.0 % (4 of 10)	40.0 % (4 of 10)
Methyl	94.0 % (47 of 50)	94.0 % (47 of 50)

1. CPx-type ATPase CopA

MLSEQKEIAM QVSGMTCAAC AARIEKGLKR MPGVTDANVN LATETVNVIY DPAETGTAAI QEKIEKLGYH VVIEGR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CPx-type ATPase CopA	[U-15N]		1.2 mM
2	phosphate			20 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 30 models in PDB: 1OQ6, Strand ID: A Detail

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Release date

2003-09-07

Citation

A core mutation affecting the folding properties of a soluble domain of the ATPase protein CopA from Bacillus subtilis
Banci, L., Bertini, I., Ciofi-Baffoni, S., Gonnelli, L., Su, X.C.
J. Mol. Biol. (2003), 331, 473-484, PubMed 12888353 , DOI: , Abstract

Related entities 1. CPx-type ATPase CopA, : 1 : 2 : 2 : 285 entities Detail

Interaction partners 1. CPx-type ATPase CopA, : 2 interactors Detail

Experiments performed 5 experiments Detail