BMRB: 5771

Solution Structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins

Authors

Muskett, F.W., May, F.E.B., Westley, B.R., Feeney, J.

Assembly

tff3 dimer

Entity

1. tff3 dimer (polymer, Thiol state: all disulfide bound), 59 monomers, 6580.397 × 2 Da Detail

EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

Total weight

13160.794 Da

Max. entity weight

6580.397 Da

Entity Connection

disulfide 7 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS57:SG	1:CYS57:SG
2	disulfide	sing	1:CYS11:SG	1:CYS37:SG
3	disulfide	sing	1:CYS11:SG	1:CYS37:SG
4	disulfide	sing	1:CYS21:SG	1:CYS36:SG
5	disulfide	sing	1:CYS21:SG	1:CYS36:SG
6	disulfide	sing	1:CYS31:SG	1:CYS48:SG
7	disulfide	sing	1:CYS31:SG	1:CYS48:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING COMBINED WITH TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 78.1 %, Completeness (bb): 90.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	78.1 % (318 of 407)	75.6 % (263 of 348)	93.2 % (55 of 59)
Backbone	90.5 % (153 of 169)	89.7 % (104 of 116)	92.5 % (49 of 53)
Sidechain	69.3 % (165 of 238)	68.5 % (159 of 232)	100.0 % (6 of 6)
Aromatic	50.0 % (16 of 32)	48.4 % (15 of 31)	100.0 % (1 of 1)
Methyl	95.5 % (21 of 22)	95.5 % (21 of 22)

1. human intestinal trefoil factor 3

EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

Show sample condition

Sample

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	human intestinal trefoil factor 3	[U-15N]		1.0 ~ 4.0 mM

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Protein Blocks Logo

Calculated from 85 models in PDB: 1E9T, Strand ID: A Detail

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Release date

2003-04-13

Citation

Solution Structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins
Muskett, F.W., May, F.E.B., Westley, B.R., Feeney, J.
Biochemistry

Related entities 1. tff3 dimer, : 1 : 3 : 1 : 37 entities Detail

Interaction partners 1. tff3 dimer, : 2 interactors Detail

Experiments performed 8 experiments Detail