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BMRB: 5773

1N8X

Solution structure of HIV-1 Stem Loop SL1

Authors

Lawrence, D.C., Stover, C.C., Noznitsky, J., Wu, Z., Summers, M.F.

Assembly

HIV-1 STEM LOOP SL1 MONOMERIC RNA

Entity

1. HIV-1 STEM LOOP SL1 MONOMERIC RNA (polymer, Thiol state: not present), 36 monomers, 11778.01 Da Detail

GGACUCGGCU UGCUGGAGAC GGCAAGAGGC GAGUCC

Formula weight

11778.01 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 100.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C
All	92.9 % (509 of 548)	87.5 % (272 of 311)	100.0 % (237 of 237)
Suger, PO4	100.0 % (396 of 396)	100.0 % (216 of 216)	100.0 % (180 of 180)
Nucleobase	74.3 % (113 of 152)	58.9 % (56 of 95)	100.0 % (57 of 57)
Aromatic	84.3 % (113 of 134)	72.7 % (56 of 77)	100.0 % (57 of 57)

1. HIV-1 Stem loop SL1

GGACUCGGCU UGCUGGAGAC GGCAAGAGGC GAGUCC

Show sample condition

Sample

Pressure 1 atm, Temperature 308 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Release date

2003-04-30

Citation

Structure of the intact stem and bulge of HIV-1 Psi-RNA stem-loop SL1
Lawrence, D.C., Stover, C.C., Noznitsky, J., Wu, Z., Summers, M.F.
J. Mol. Biol. (2003), 326, 529-542, PubMed 12559920 , DOI: , Abstract

Related entities 1. HIV-1 STEM LOOP SL1 MONOMERIC RNA, : 1 : 1 : 1 entities Detail

Experiments performed 6 experiments Detail

1 3D 13C-separated NOESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

2 4D 13C-separated NOESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

3 3D 13C-separated ROESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

4 IPAP H-COUPLED CT-HSQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

5 2D NOESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

6 2D ROESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
1	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
2	Tris-Cl		10 mM
3	Na-EDTA		0.1 mM
4	D2O		100 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
5	HIV-1 Stem loop SL1	[13C, 15N]-cytidine	1.0 mM
6	Tris-Cl		10 mM
7	Na-EDTA		0.1 mM
8	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
9	HIV-1 Stem loop SL1	1.0 mM
10	Tris-Cl	10 mM
11	Na-EDTA	0.1 mM
12	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Isotope labeling	Concentration
13	HIV-1 Stem loop SL1	[U-13C; U-15N]	1.0 mM
14	Tris-Cl		10 mM
15	Na-EDTA		0.1 mM
16	Pf1 phage		17 mg/mL

Sample #5

Pressure 1 atm, Temperature 308 (±0.2) K

#	Name	Concentration
17	HIV-1 Stem loop SL1	1.0 mM
18	Tris-Cl	10 mM
19	Na-EDTA	0.1 mM
20	D2O	10 %
21	H2O	90 %

nullKeywords BULGE, G-A MISMATCH, HIV, RNA STEM LOOP, SL1

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Found 1 Document (1.363 seconds)

BMRB: 5773

Sample

Related entities 1. HIV-1 STEM LOOP SL1 MONOMERIC RNA, : 1 : 1 : 1 entities Detail

Experiments performed 6 experiments Detail

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