BMRB: 5775

5'(dCCUCCUU)3':3'(rAGGAGGAAA)5'

Authors

Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.

Assembly

5'-R(*AP*AP*AP*GP*GP*AP*GP*GP*A)-3'/5'-D(*CP*CP*UP*CP*CP*UP*U)-3'

Entity

1. RNA chain (polymer, Thiol state: not present), 11 monomers, 3696.238 Da Detail

UAAAGGAGGA G

2. DNA chain (polymer, Thiol state: not present), 7 monomers, 2045.242 Da Detail

CCXCCXX

Total weight

5741.48 Da

Max. entity weight

3696.238 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 27.8 %, Completeness: 32.3 %, Completeness (bb): 36.2 % Detail

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Polymer type: polyribonucleotide polydeoxyribonucleotide

	Total	1H
All	32.3 % (43 of 133)	32.3 % (43 of 133)
Suger, PO4	36.2 % (34 of 94)	36.2 % (34 of 94)
Nucleobase	23.1 % (9 of 39)	23.1 % (9 of 39)
Aromatic	29.0 % (9 of 31)	29.0 % (9 of 31)

1. RNA chain

UAAAGGAGGA G

2. DNA chain

CCXCCX

Show sample condition

Sample

Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	RNA chain			1 mM
2	DNA chain			1 mM
3	NaCl			80 mM
4	phosphate buffer			10 mM
5	EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

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Chem. Shift Complete2

Sequence coverage: 61.1 %, Completeness: 47.4 %, Completeness (bb): 53.2 % Detail

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Polymer type: polyribonucleotide polydeoxyribonucleotide

	Total	1H
All	47.4 % (126 of 266)	47.4 % (126 of 266)
Suger, PO4	53.2 % (100 of 188)	53.2 % (100 of 188)
Nucleobase	33.3 % (26 of 78)	33.3 % (26 of 78)
Aromatic	41.9 % (26 of 62)	41.9 % (26 of 62)

1. RNA chain

UAAAGGAGGA G

2. DNA chain

CCXCCX

Show sample condition

Sample

Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	RNA chain			1 mM
2	DNA chain			1 mM
3	NaCl			80 mM
4	phosphate buffer			10 mM
5	EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

Hide sample codition

Release date

2003-06-08

Citation

NMR studies of DNA single strands and DNA:RNA hybrids with and without 1-propynylation at C5 of oligopyrimidines
Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.
J. Am. Chem. Soc. (2003), 125, 6090-6097, PubMed 12785839 , DOI 10.1021/ja021285d , Abstract

Experiments performed 1 experiments Detail