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BMRB: 5776


5776
1NTS
sPrODN1:RNA [5'-R(AAAGGAGGA)-3'/5'-D(CXXXXXX)-3']
Authors
Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.
Assembly
sPrODN1:RNA
Entity
1. sPrODN1 chain (polymer, Thiol state: not present), 11 monomers, 3696.238 Da Detail

UAAAGGAGGA G


2. DNA chain (polymer, Thiol state: not present), 7 monomers, 2285.626 Da Detail

CXXXXXX


Total weight
5981.8643 Da
Max. entity weight
3696.238 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 5.6 %, Completeness: 8.0 %, Completeness (bb): 8.2 % Detail
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Polymer type: polyribonucleotide polydeoxyribonucleotide

Total1H
All 8.0 % (8 of 100) 8.0 % (8 of 100)
Suger, PO4 8.2 % (6 of 73) 8.2 % (6 of 73)
Nucleobase 7.4 % (2 of 27) 7.4 % (2 of 27)
Aromatic 8.0 % (2 of 25) 8.0 % (2 of 25)

1. sPrODN1 chain

UAAAGGAGGA G

2. DNA chain

CXXX

Show sample condition
Sample

Pressure 1 atm, Temperature 273 K, pH 7.0


#NameIsotope labelingTypeConcentration
1sPrODN1 chain1 mM
2DNA chain1 mM
3NaCl80 mM
4phosphate buffer10 mM
5EDTA0.5 mM
6H2O90 %
7D2O10 %

Chem. Shift Complete2
Sequence coverage: 61.1 %, Completeness: 44.0 %, Completeness (bb): 47.9 % Detail
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Polymer type: polyribonucleotide polydeoxyribonucleotide

Total1H
All44.0 % (88 of 200)44.0 % (88 of 200)
Suger, PO447.9 % (70 of 146)47.9 % (70 of 146)
Nucleobase33.3 % (18 of 54)33.3 % (18 of 54)
Aromatic36.0 % (18 of 50)36.0 % (18 of 50)

1. sPrODN1 chain

UAAAGGAGGA G

2. DNA chain

CXXX

Show sample condition
Sample

Pressure 1 atm, Temperature 273 K, pH 7.0


#NameIsotope labelingTypeConcentration
1sPrODN1 chain1 mM
2DNA chain1 mM
3NaCl80 mM
4phosphate buffer10 mM
5EDTA0.5 mM
6H2O90 %
7D2O10 %

Release date
2003-06-08
Citation
NMR studies of DNA single strands and DNA:RNA hybrids with and without 1-propynylation at C5 of oligopyrimidines
Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.
J. Am. Chem. Soc. (2003), 125, 6090-6097, PubMed 12785839 , DOI 10.1021/ja021285d ,
Experiments performed 1 experiments Detail
nullKeywords DNA, hybrid, propynyl, RNA