BMRB: 5777

PODN:RNA [5'-R(AAAGGAGGA)-3'/5'-D(XXXXXXX)-3']

Authors

Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.

Assembly

PODN:RNA

Entity

1. PODN chain (polymer, Thiol state: not present), 11 monomers, 3696.238 Da Detail

UAAAGGAGGA G

2. DNA chain (polymer, Thiol state: not present), 7 monomers, 2323.674 Da

Total weight

6019.912 Da

Max. entity weight

3696.238 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 0.0 %, Completeness: 0.0 %, Completeness (bb): 0.0 % Detail

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Polymer type: polyribonucleotide polydeoxyribonucleotide

	Total	1H
All	0.0 % (0 of 89)	0.0 % (0 of 89)
Suger, PO4	0.0 % (0 of 66)	0.0 % (0 of 66)
Nucleobase	0.0 % (0 of 23)	0.0 % (0 of 23)
Aromatic	0.0 % (0 of 23)	0.0 % (0 of 23)

1. PODN chain

UAAAGGAGGA G

Show sample condition

Sample

Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	PODN chain			1 mM
2	DNA chain			1 mM
3	NaCl			80 mM
4	phosphate buffer			10 mM
5	EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

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Chem. Shift Complete2

Sequence coverage: 61.1 %, Completeness: 46.1 %, Completeness (bb): 50.0 % Detail

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Polymer type: polyribonucleotide polydeoxyribonucleotide

	Total	1H
All	46.1 % (82 of 178)	46.1 % (82 of 178)
Suger, PO4	50.0 % (66 of 132)	50.0 % (66 of 132)
Nucleobase	34.8 % (16 of 46)	34.8 % (16 of 46)
Aromatic	34.8 % (16 of 46)	34.8 % (16 of 46)

1. PODN chain

UAAAGGAGGA G

Show sample condition

Sample

Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	PODN chain			1 mM
2	DNA chain			1 mM
3	NaCl			80 mM
4	phosphate buffer			10 mM
5	EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

Hide sample codition

Release date

2003-06-08

Citation

NMR studies of DNA single strands and DNA:RNA hybrids with and without 1-propynylation at C5 of oligopyrimidines
Znosko, B.M., Barnes, T.W., Krugh, T.R., Turner, D.H.
J. Am. Chem. Soc. (2003), 125, 6090-6097, PubMed 12785839 , DOI 10.1021/ja021285d , Abstract

Experiments performed 1 experiments Detail