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BMRB: 5779

1NNV

The Solution structure of HI1450

Authors

Parsons, L.M., Yeh, D.CHEON., Orban, J.

Assembly

HYPOTHETICAL PROTEIN HI1450

Entity

1. HYPOTHETICAL PROTEIN HI1450 (polymer, Thiol state: not present), 110 monomers, 12806.03 Da Detail

GSHMTTEIKK LDPDTAIDIA YDIFLEMAGE NLDPADILLF NLQFEERGGV EFVETADDWE EEIGVLIDPE EYAEVWVGLV NEQDEMDDVF AKFLISHREE DREFHVIWKK

Formula weight

12806.03 Da

Source organism

Haemophilus influenzae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 91.3 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1186 of 1299)	96.7 % (647 of 669)	83.1 % (428 of 515)	96.5 % (111 of 115)
Backbone	95.7 % (626 of 654)	97.3 % (217 of 223)	94.1 % (305 of 324)	97.2 % (104 of 107)
Sidechain	88.3 % (661 of 749)	96.4 % (430 of 446)	75.9 % (224 of 295)	87.5 % (7 of 8)
Aromatic	52.2 % (70 of 134)	97.0 % (65 of 67)	3.1 % (2 of 64)	100.0 % (3 of 3)
Methyl	93.7 % (118 of 126)	95.2 % (60 of 63)	92.1 % (58 of 63)

1. HYPOTHETICAL PROTEIN HI1450

GSHMTTEIKK LDPDTAIDIA YDIFLEMAGE NLDPADILLF NLQFEERGGV EFVETADDWE EEIGVLIDPE EYAEVWVGLV NEQDEMDDVF AKFLISHREE DREFHVIWKK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.1)

#	Name	Isotope labeling	Concentration
1	HYPOTHETICAL PROTEIN HI1450	[U-13C; U-15N]	1.0 mM
2	phosphate		50 mM
3	NaCl		100 mM
4	EDTA		0.5 mM
5	H20		90 %
6	D20		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.7 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.7 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.78 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.46 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1NNV, Strand ID: A Detail

Release date

2004-03-06

Citation

Solution structure of the highly acidic protein HI1450 from Haemophilus influenzae, a putative double-stranded DNA mimic
Parsons, L.M., Yeh, D.CHEON., Orban, J.
Proteins (2004), 54, 375-383, PubMed 14747986 , DOI 10.1002/prot.10607 , Abstract

Related entities 1. HYPOTHETICAL PROTEIN HI1450, : 1 : 2 : 34 entities Detail

Experiments performed 11 experiments Detail

1 3D 13C-separated NOESY