BMRB: 5780

NMR structure of the PYRIN domain of human ASC

Authors

Liepinsh, E., Barbals, R., Dahl, E., Sharipo, A., Staub, E., Otting, G.

Assembly

apoptosis-associated SPECK-like peotein containing a CARD

Entity

1. apoptosis-associated SPECK-like peotein containing a CARD (polymer, Thiol state: not present), 103 monomers, 11478.04 Da Detail

MRGSHHHHHH GSMGRARDAI LDALENLTAE ELKKFKLKLL SVPLREGYGR IPRGALLSMD ALDLTDKLVS FYLETYGAEL TANVLRDMGL QEMAGQLQAA THQ

Formula weight

11478.04 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 87.9 %, Completeness (bb): 88.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.9 % (537 of 611)	87.9 % (537 of 611)
Backbone	88.3 % (188 of 213)	88.3 % (188 of 213)
Sidechain	87.7 % (349 of 398)	87.7 % (349 of 398)
Aromatic	66.7 % (24 of 36)	66.7 % (24 of 36)
Methyl	100.0 % (62 of 62)	100.0 % (62 of 62)

1. PYRIN domain

MRGSHHHHHH GSMGRARDAI LDALENLTAE ELKKFKLKLL SVPLREGYGR IPRGALLSMD ALDLTDKLVS FYLETYGAEL TANVLRDMGL QEMAGQLQAA THQ

Show sample condition

Sample

Temperature 301 (±0.5) K, pH 3.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PYRIN domain			1.0 mM
2	NaCl			0.05 M

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UCP, Strand ID: A Detail

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Release date

2004-05-14

Citation

The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition
Liepinsh, E., Barbals, R., Dahl, E., Sharipo, A., Staub, E., Otting, G.
J. Mol. Biol. (2003), 332, 1155-1163, PubMed 14499617 , DOI 10.1016/j.jmb.2003.07.007 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. apoptosis-associated SPECK-like peotein containing a CARD, : 1 : 5 : 57 entities Detail

Interaction partners 1. apoptosis-associated SPECK-like peotein containing a CARD, : 28 interactors Detail

Experiments performed 4 experiments Detail