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BMRB: 5781


5781
1OO7
The Solution Structure of a DNA.RNA Duplex Containing 5-Propynyl U and C Comparison with 5-Me Modifications
Authors
Gyi, J.I., Gao, D., Conn, G.L., Trent, J.O., Brown, T., Lane, A.N.
Assembly
Propynyl DNA.RNA hybrid
Entity
1. DNA.RNA hybrid (polymer, Thiol state: not present), 10 monomers, 2954.781 Da Detail

GCXXCXCXXC


2. DNA.RNA hybrid (polymer), 10 monomers, 3350.048 Da Detail

GAAGAGAAGC


Total weight
6304.829 Da
Max. entity weight
3350.048 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 25.0 %, Completeness: 34.1 %, Completeness (bb): 30.5 % Detail
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Polymer type: polydeoxyribonucleotide polyribonucleotide

Total1H
All34.1 % (46 of 135)34.1 % (46 of 135)
Suger, PO430.5 % (29 of 95)30.5 % (29 of 95)
Nucleobase42.5 % (17 of 40)42.5 % (17 of 40)
Aromatic30.0 % (9 of 30)30.0 % (9 of 30)

1. DNA.RNA hybrid

GCXXCXCXXC

2. DNA.RNA hybrid

GAAGAGAAGC

Show sample condition
Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1DNA.RNA_hybrid1 mM
2DNA.RNA_hybrid1 mM
3D2O100 %
4KCL100 mM

Chem. Shift Complete2
Sequence coverage: 50.0 %, Completeness: 42.2 %, Completeness (bb): 40.0 % Detail
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Polymer type: polydeoxyribonucleotide polyribonucleotide

Total1H
All42.2 % (114 of 270)42.2 % (114 of 270)
Suger, PO440.0 % (76 of 190)40.0 % (76 of 190)
Nucleobase47.5 % (38 of 80)47.5 % (38 of 80)
Aromatic46.7 % (28 of 60)46.7 % (28 of 60)

1. DNA.RNA hybrid

GCXXCXCXXC

2. DNA.RNA hybrid

GAAGAGAAGC

Show sample condition
Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1DNA.RNA_hybrid1 mM
2DNA.RNA_hybrid1 mM
3D2O100 %
4KCL100 mM

Release date
2003-05-18
Citation
The solution structure of a DNA*RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications
Gyi, J.I., Gao, D., Conn, G.L., Trent, J.O., Brown, T., Lane, A.N.
Nucleic Acids Res. (2003), 31, 2683-2693, PubMed 12736318 ,
Experiments performed 3 experiments Detail
nullKeywords ANTISENSE, DNA.RNA HYBRID, PURINE/PYRIMIDINE-RICH STRANDS