BMRB: 5786

Solution Structure of Neural Cadherin Prodomain

Authors

Koch, A.W., Farooq, A., Shan, W., Gruzglin, G., Zeng, L., Colman, D.R., Zhou, M.

Assembly

Neural cadherin

Entity

1. Neural cadherin (polymer, Thiol state: all disulfide bound), 159 monomers, 18301.09 Da Detail

GHHHHHHHHH HSSGHIDDDD KHMEASGEIA LCKTGFPEDV YSAVLPKDVH EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLTAEQA KFLIYAQDKE TQEKWQVAVN LSREPTLTEE PMKEPHEIEE IVFPRQLAKH SGALQRQKR

Formula weight

18301.09 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS32:SG	1:CYS63:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.0 %, Completeness: 59.4 %, Completeness (bb): 68.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	59.4 % (1110 of 1869)	55.1 % (540 of 980)	62.1 % (450 of 725)	73.2 % (120 of 164)
Backbone	68.1 % (637 of 936)	74.1 % (234 of 316)	60.4 % (284 of 470)	79.3 % (119 of 150)
Sidechain	55.3 % (600 of 1085)	46.1 % (306 of 664)	72.0 % (293 of 407)	7.1 % (1 of 14)
Aromatic	32.3 % (53 of 164)	32.9 % (27 of 82)	30.9 % (25 of 81)	100.0 % (1 of 1)
Methyl	81.9 % (113 of 138)	78.3 % (54 of 69)	85.5 % (59 of 69)

1. N-cadherin prodomain

GHHHHHHHHH HSSGHIDDDD KHMEASGEIA LCKTGFPEDV YSAVLPKDVH EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLTAEQA KFLIYAQDKE TQEKWQVAVN LSREPTLTEE PMKEPHEIEE IVFPRQLAKH SGALQRQKR

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	N-cadherin prodomain	[U-13C; U-15N]		1 mM
2	Phosphate			50 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 1OP4, Strand ID: A Detail

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Release date

2004-02-10

Citation

1H, 13C and 15N resonance assignments for the N-cadherin prodomain
Koch, A.W., Farooq, A., Zeng, L., Colman, D.R., Zhou, M.
J. Biomol. NMR (2004), 28, 87-88, PubMed 14739643 , DOI 10.1023/B:JNMR.0000012831.75102.d3 , Abstract

Related entities 1. Neural cadherin, : 1 : 32 entities Detail

Interaction partners 1. Neural cadherin, : 22 interactors Detail

Experiments performed 3 experiments Detail