BMRBj - BMREntryMaster

Found 1 Document (1.694 seconds)

BMRB: 5787

1Z5F

1H, 13C and 15N resonance assignments and secondary structure of the cytotoxic protein RNase 3 from oocytes of bullfrog Rana catesbeiana

Authors

Lou, Y., Pan, Y., Ho, Y., Liao, Y., Chen, C.

Assembly

RC-RNase 3

Entity

1. RC-RNase 3 (polymer, Thiol state: all disulfide bound), 105 monomers, 11875.75 Da Detail

XDWETFQKKH LTDTKKVKCD VEMAKALFDC KKTNTFIYAL PGRVKALCKN IRDNTDVLSR DAFLLPQCDR IKLPCHYKLS SSTNTICITC VNQLPIHFAG VGSCP

Formula weight

11875.75 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS19:SG	1:CYS68:SG
2	disulfide	sing	1:CYS30:SG	1:CYS75:SG
3	disulfide	sing	1:CYS48:SG	1:CYS90:SG
4	disulfide	sing	1:CYS87:SG	1:CYS104:SG

Source organism

Lithobates catesbeianus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 97.7 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (1205 of 1233)	96.7 % (621 of 642)	98.8 % (477 of 483)	99.1 % (107 of 108)
Backbone	98.4 % (604 of 614)	97.1 % (200 of 206)	99.0 % (306 of 309)	99.0 % (98 of 99)
Sidechain	97.6 % (703 of 720)	96.6 % (421 of 436)	99.3 % (273 of 275)	100.0 % (9 of 9)
Aromatic	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (44 of 44)	100.0 % (1 of 1)
Methyl	99.2 % (117 of 118)	98.3 % (58 of 59)	100.0 % (59 of 59)

1. RC-RNase 3

XDWETFQKKH LTDTKKVKCD VEMAKALFDC KKTNTFIYAL PGRVKALCKN IRDNTDVLSR DAFLLPQCDR IKLPCHYKLS SSTNTICITC VNQLPIHFAG VGSCP

Show sample condition

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.6 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.6 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.69 ppm, Outliers: 6 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1Z5F, Strand ID: A Detail

Release date

2003-09-04

Citation

1H, 13C and 15N resonance assignments and secondary structure of the cytotoxic RNase 3 from oocytes of bullfrog Rana catesbeiana
Lou, Y., Pan, Y., Ho, Y., Liao, Y., Chen, C.
J. Biomol. NMR (2003), 27, 289-290, PubMed 12975591 , DOI: , Abstract

Related entities 1. RC-RNase 3, : 1 : 77 entities Detail

Experiments performed 14 experiments Detail

1 2D TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

2 2D DQF-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

3 2D NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

4 2D 1H-13C HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

5 2D 1H-15N HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

6 3D HNCACB

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

7 3D CBCA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

8 3D HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

9 3D HN(CA)CO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

10 3D 15N NOESY-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

11 3D 15N TOCSY-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

12 3D C(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

13 3D HC(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

14 3D HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker Avance (500 MHz)

Sample #1

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RC-RNase 3	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 310 (±2) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	RC-RNase 3			1.5 mM

nullKeywords antitumor, bullfrog, cytotoxicity, lectin, ribonuclease

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Found 1 Document (1.694 seconds)

BMRB: 5787

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Related entities 1. RC-RNase 3, : 1 : 77 entities Detail

Experiments performed 14 experiments Detail

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