BMRBj - BMREntryMaster

Found 1 Document (1.738 seconds)

BMRB: 5796

1PA4

Solution Structure of a Putative Ribosome Binding-Protein from Mycoplasma pneumoniae and Comparison to a Distant Homolog

Authors

Rubin, S.M., Pelton, J.G., Yokota, H., Kim, R., Wemmer, D.E.

Assembly

MPN156

Entity

1. MPN156 (polymer, Thiol state: all free), 117 monomers, 13446.38 Da Detail

GMASYKKERL ENDIIRLINR TVIHEIYNET VKTGHVTHVK LSDDLLHVTV YLDCYNREQI DRVVGAFNQA KGVFSRVLAH NLYLAKAVQI HFVKDKAIDN AMRIESIINS LKKSKPN

Formula weight

13446.38 Da

Source organism

Mycoplasma pneumoniae

Exptl. method

NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 62.0 %, Completeness (bb): 70.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.0 % (872 of 1406)	61.9 % (452 of 730)	59.3 % (325 of 548)	74.2 % (95 of 128)
Backbone	70.1 % (491 of 700)	79.7 % (189 of 237)	59.7 % (207 of 347)	81.9 % (95 of 116)
Sidechain	57.8 % (473 of 819)	53.3 % (263 of 493)	66.9 % (210 of 314)	0.0 % (0 of 12)
Aromatic	34.0 % (32 of 94)	34.0 % (16 of 47)	34.0 % (16 of 47)
Methyl	79.7 % (126 of 158)	81.0 % (64 of 79)	78.5 % (62 of 79)

1. MPN156

GMASYKKERL ENDIIRLINR TVIHEIYNET VKTGHVTHVK LSDDLLHVTV YLDCYNREQI DRVVGAFNQA KGVFSRVLAH NLYLAKAVQI HFVKDKAIDN AMRIESIINS LKKSKPN

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.73 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.73 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.73 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 1PA4, Strand ID: A Detail

Coupling constant

65 J values in 1 lists
Temperature 298 (±1) K, pH 6.6 (±0.1) Detail

Release date

2004-10-17

Citation

Solution structure of a putative ribosome binding protein from Mycoplasma pneumoniae and comparison to a distant homolog
Rubin, S.M., Pelton, J.G., Yokota, H., Kim, R., Wemmer, D.E.
J. Struct. Funct. Genomics (2003), 4, 235-243, PubMed 15185964 , DOI 10.1023/b:jsfg.0000016127.57320.82 , Abstract

Related entities 1. MPN156, : 1 : 1 : 68 entities Detail

Interaction partners 1. MPN156, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

2 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

3 HNCACB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

5 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

6 HNHA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

7 15-N NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

8 HCCH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	MPN156	[U-100% 15N]	1 mM
2	sodium phosphate		25 mM
3	sodium chloride		50 mM
4	DTT		1 mM
5	EDTA		1 mM

Sample #2

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	MPN156	[U-100% 15N; U-100% 13C]	1 mM
7	sodium phosphate		25 mM
8	sodium chloride		50 mM
9	DTT		1 mM
10	EDTA		1 mM

Sample #3

Temperature 298 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	MPN156	[U-10% 13C]	1 mM
12	sodium phosphate		25 mM
13	sodium chloride		50 mM
14	DTT		1 mM
15	EDTA		1 mM

null

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Found 1 Document (1.738 seconds)

BMRB: 5796

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MPN156, : 1 : 1 : 68 entities Detail

Interaction partners 1. MPN156, : 2 interactors Detail

Experiments performed 8 experiments Detail

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