BMRB: 5798

Solution structure of Protein yrbA from Escherichia Coli: Northeast Structural Genomics Consortium target ER115

Authors

Gurla, S.V.T., Huang, J.Y., Acton, T.B., Shastry, R., Chiang, Y., Montelione, G.T.

Assembly

Protein yrbA

Entity

1. Protein yrbA (polymer, Thiol state: not present), 97 monomers, 11102.40 Da Detail

MIEDPMENNE IQSVLMNALS LQEVHVSGDG SHFQVIAVGE LFDGMSRVKK QQTVYGPLME YIADNRIHAV SIKAYTPAEW ARDRKLNGFL EHHHHHH

Formula weight

11102.4 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 91.3 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.3 % (1034 of 1133)	92.7 % (544 of 587)	88.7 % (391 of 441)	94.3 % (99 of 105)
Backbone	92.4 % (532 of 576)	94.9 % (187 of 197)	90.5 % (258 of 285)	92.6 % (87 of 94)
Sidechain	89.4 % (579 of 648)	90.3 % (352 of 390)	87.4 % (216 of 247)	100.0 % (11 of 11)
Aromatic	64.7 % (66 of 102)	66.7 % (34 of 51)	62.0 % (31 of 50)	100.0 % (1 of 1)
Methyl	96.1 % (98 of 102)	96.1 % (49 of 51)	96.1 % (49 of 51)

1. yrbA

MIEDPMENNE IQSVLMNALS LQEVHVSGDG SHFQVIAVGE LFDGMSRVKK QQTVYGPLME YIADNRIHAV SIKAYTPAEW ARDRKLNGFL EHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	yrbA	[U-13C; U-15N]		0.8 mM
2	NH4OAc			20 mM
3	NaCl			100 mM
4	CaCl2			5 mM
5	DTT			10 mM
6	azide			0.02 %
7	H2O			95 %
8	D2O			5 %

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LACS Plot; CA

Referencing offset: -0.48 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

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LACS Plot; CB

Referencing offset: -0.48 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

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LACS Plot; CO

Referencing offset: 1.93 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.1013291

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Protein Blocks Logo

Calculated from 16 models in PDB: 1NY8, Strand ID: A Detail

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Release date

2006-04-05

Citation

Solution structure of Protein yrbA from Escherichia Coli: Northeast Structural Genomics Consortium target ER115
Gurla, S.V.T., Huang, J.Y., Acton, T.B., Shastry, R., Chiang, Y., Montelione, G.T.

Related entities 1. Protein yrbA, : 1 : 2 : 14 entities Detail

Experiments performed 2 experiments Detail