BMRB: 5807

Letter to the Editor: Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD

Authors

Iwai, H.

Assembly

gpD

Entity

1. gpD (polymer, Thiol state: not present), 109 monomers, 11440.58 Da Detail

TSKETFTHYQ PQGNSDPAHT ATAPGGLSAK APAMTPLMLD TSSRKLVAWD GTTDGAAVGI LAVAADQTST TLTFYKSGTF RYEDVLWPEA ASDETKKRTA FAGTAISIV

Formula weight

11440.58 Da

Source organism

Escherichia virus Lambda

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 95.0 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	95.0 % (665 of 700)	94.8 % (560 of 591)	96.3 % (105 of 109)
Backbone	96.3 % (311 of 323)	96.4 % (212 of 220)	96.1 % (99 of 103)
Sidechain	93.9 % (354 of 377)	93.8 % (348 of 371)	100.0 % (6 of 6)
Aromatic	88.0 % (44 of 50)	87.5 % (42 of 48)	100.0 % (2 of 2)
Methyl	98.4 % (63 of 64)	98.4 % (63 of 64)

1. bacteriophage lambda gpD

TSKETFTHYQ PQGNSDPAHT ATAPGGLSAK APAMTPLMLD TSSRKLVAWD GTTDGAAVGI LAVAADQTST TLTFYKSGTF RYEDVLWPEA ASDETKKRTA FAGTAISIV

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	bacteriophage lambda gpD	[U-98% 15N]		1.5 mM
2	potassium phosphate			20.0 ~ 50.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1VD0, Strand ID: A Detail

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Release date

2004-02-10

Citation 1

Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD
Iwai, H., Forrer, P., Pluckthun, A., Guntert, P.
J. Biomol. NMR (2004), 28, 89-90, PubMed 14739644 , DOI 10.1023/B:JNMR.0000012844.21707.8c , Abstract

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Citation 2

Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD
Yang, F., Forrer, P., Dauter, Z., Conway, J.F., Cheng, N., Cerritelli, M.E., Steven, A.C., Pluckthun, A., Wlodawer, A.
Nat. Struct. Biol. (2000), 7, 230-237, PubMed 10700283 , DOI 10.1038/73347 , Abstract

Hide non-primary 1 citaion

Related entities 1. gpD, : 1 : 6 : 3 : 5 entities Detail

Interaction partners 1. gpD, : 2 interactors Detail