BMRBj - BMREntryMaster

Found 1 Document (2.137 seconds)

BMRB: 5817

1PB5

NMR Structure of a Prototype LNR Module from Human Notch1

Authors

Vardar, D., North, C.L., Sanchez-Irizarry, C., Aster, J.C., Blacklow, S.C.

Assembly

Neurogenic locus notch homolog protein 1

Entity

1. Neurogenic locus notch homolog protein 1 (polymer, Thiol state: all disulfide bound), 35 monomers, 3715.946 Da Detail

EEACELPECQ EDAGNKVCSL QCNNHACGWD GGDCS

2. CA (non-polymer), 40.078 Da

Total weight

3756.024 Da

Max. entity weight

3715.946 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS27:SG
2	disulfide	sing	1:CYS9:SG	1:CYS22:SG
3	disulfide	sing	1:CYS18:SG	1:CYS34:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.9 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.9 % (286 of 349)	90.6 % (164 of 181)	64.1 % (82 of 128)	100.0 % (40 of 40)
Backbone	82.7 % (172 of 208)	100.0 % (73 of 73)	64.4 % (65 of 101)	100.0 % (34 of 34)
Sidechain	84.3 % (145 of 172)	84.3 % (91 of 108)	82.8 % (48 of 58)	100.0 % (6 of 6)
Aromatic	56.3 % (9 of 16)	100.0 % (8 of 8)	0.0 % (0 of 7)	100.0 % (1 of 1)
Methyl	83.3 % (15 of 18)	66.7 % (6 of 9)	100.0 % (9 of 9)

1. Neurogenic locus notch homolog protein 1

EEACELPECQ EDAGNKVCSL QCNNHACGWD GGDCS

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

Protein Blocks Logo

Calculated from 16 models in PDB: 1PB5, Strand ID: A Detail

Release date

2003-06-03

Citation

Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1
Vardar, D., North, C.L., Sanchez-Irizarry, C., Aster, J.C., Blacklow, S.C.
Biochemistry (2003), 42, 7061-7067, PubMed 12795601 , DOI 10.1021/bi034156y , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P46531 released on 1995-11-01
Title NOTC1_HUMAN Entity Neurogenic locus notch homolog protein 1

Related entities 1. Neurogenic locus notch homolog protein 1, : 1 : 4 : 3 : 1 : 39 entities Detail

Interaction partners 1. Neurogenic locus notch homolog protein 1, : 33 interactors Detail

More details for 33 interactors identified by 18 citations

Experiments performed 7 experiments Detail

1 HNCA

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

2 HNCOCA

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

3 13C HSQC

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

4 3D 15N-separated TOCSY

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

5 2D TOCSY

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

6 15N-HSQC

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

7 15N-HMQC-J

Instrument

Model Varian UNITY (400 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	Neurogenic locus notch homolog protein 1	[U-15N]	1 mM
2	calcium salt		10 mM
3	DSS		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	Neurogenic locus notch homolog protein 1	[U-13C; U-15N]	1 mM
7	calcium salt		10 mM
8	DSS		0.5 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
11	Neurogenic locus notch homolog protein 1	[U-15N]	1.5 mM
12	calcium salt		10 mM
13	DSS		0.5 mM
14	PIPES	[U-2H]	50 mM
15	H2O		90 %
16	D2O		10 %

nullKeywords LIN12/Notch repeat, Notch signaling, calcium-binding domain, disulfide bond, protein module

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Found 1 Document (2.137 seconds)

BMRB: 5817

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Neurogenic locus notch homolog protein 1, : 1 : 4 : 3 : 1 : 39 entities Detail

Interaction partners 1. Neurogenic locus notch homolog protein 1, : 33 interactors Detail

Experiments performed 7 experiments Detail

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