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Found 1 Document (1.965 seconds)

BMRB: 5818

1P9K

The solution structure of YbcJ from E. coli reveals a recently discovered alfaL motif involved in RNA-binding

Authors: Gandhi, S., Volpon, L., Lievre, C., Osborne, M.J., Iannuzzi, P., Larocque, R., Matte, A., Cygler, M., Gehring, K., Ekiel, I.
Assembly: YbcJ
Entity: 1. YbcJ (polymer, Thiol state: all free), 79 monomers, 8403.629 Da Detail
GSMIHRMSNM ATFSLGKHPH VELCDLLKLE GWSESGAQAK IAIAEGQVKV DGAVETRKRC KIVAGQTVSF AGHSVQVVA
Formula weight: 8403.629 Da
Source organism: Escherichia coli
Exptl. method: NMR
Refine. method: Simulated annealing
Data set: heteronucl_NOEs
Protein Blocks Logo: Calculated from 20 models in PDB: 1P9K, Strand ID: A Detail
Heteronucl. NOE: 51 NOE values in 1 lists
Value type na, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 313 K, pH 6.8 Detail
Pressure 1 atm, Temperature 313 K, pH 6.8
Release date: 2003-09-11
Citation: The solution structure of YbcJ from Escherichia coli reveals a recently discovered alphaL motif involved in RNA binding
Volpon, L., Lievre, C., Osborne, M.J., Gandhi, S., Iannuzzi, P., Larocque, R., Cygler, M., Gehring, K., Ekiel, I.
J. Bacteriol. (2003), 185, 4204-4210, PubMed 12837795 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P0AAS7 released on 2005-10-11
Title YBCJ_ECOLI Entity Uncharacterized protein YbcJ

Related entities 1. YbcJ, : 1 : 2 : 1 entities Detail

Interaction partners 1. YbcJ, : 10 interactors Detail

More details for 10 interactors identified by 2 citations

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
2	phosphate buffer		50 mM
3	NaCl		300 mM
4	DTT		15 mM
5	sodium azide		1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
9	phosphate buffer		50 mM
10	NaCl		300 mM
11	DTT		15 mM
12	sodium azide		1 mM
13	Pf1 phage		8 mg/mL
14	H2O		90 %
15	D2O		10 %

2 DQF-COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
2	phosphate buffer		50 mM
3	NaCl		300 mM
4	DTT		15 mM
5	sodium azide		1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
9	phosphate buffer		50 mM
10	NaCl		300 mM
11	DTT		15 mM
12	sodium azide		1 mM
13	Pf1 phage		8 mg/mL
14	H2O		90 %
15	D2O		10 %

3 HNHA

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
2	phosphate buffer		50 mM
3	NaCl		300 mM
4	DTT		15 mM
5	sodium azide		1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
9	phosphate buffer		50 mM
10	NaCl		300 mM
11	DTT		15 mM
12	sodium azide		1 mM
13	Pf1 phage		8 mg/mL
14	H2O		90 %
15	D2O		10 %

4 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
2	phosphate buffer		50 mM
3	NaCl		300 mM
4	DTT		15 mM
5	sodium azide		1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
9	phosphate buffer		50 mM
10	NaCl		300 mM
11	DTT		15 mM
12	sodium azide		1 mM
13	Pf1 phage		8 mg/mL
14	H2O		90 %
15	D2O		10 %

5 IPAP-HSQ

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
2	phosphate buffer		50 mM
3	NaCl		300 mM
4	DTT		15 mM
5	sodium azide		1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 313 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	YbcJ	[U-13C; U-15N]	4.0 ~ 7.0 mM
9	phosphate buffer		50 mM
10	NaCl		300 mM
11	DTT		15 mM
12	sodium azide		1 mM
13	Pf1 phage		8 mg/mL
14	H2O		90 %
15	D2O		10 %

nullKeywords alfaL motif, RNA-binding protein, E. coli

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Found 1 Document (1.965 seconds)

BMRB: 5818

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. YbcJ, : 1 : 2 : 1 entities Detail

Interaction partners 1. YbcJ, : 10 interactors Detail

Experiments performed 5 experiments Detail

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