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Found 1 Document (1.662 seconds)

BMRB: 5824

1Q6A

Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein (oxidized form)

Authors

Vakonakis, I., LiWang, A.C.

Assembly

KaiA C-terminal domain

Entity

1. KaiA C-terminal domain (polymer, Thiol state: all disulfide bound), 107 monomers, 12583.44 × 2 Da Detail

AMARMSPADK RKLLDELRSI YRTIVLEYFN TDAKVNERID EFVSKAFFAD ISVSQVLEIH VELMDTFSKQ LKLEGRSEDI LLDYRLTLID VIAHLCEMYR RSIPREV

Total weight

25166.88 Da

Max. entity weight

12583.44 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS96:SG	1:CYS96:SG

Source organism

Thermosynechococcus elongatus

Exptl. method

NMR

Refine. method

Distance geometry, Simulated Annealing, Radius-of-gyration, Carbon chemical shift and conformational database potential refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.1 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.1 % (1143 of 1297)	94.4 % (637 of 675)	78.9 % (405 of 513)	92.7 % (101 of 109)
Backbone	82.4 % (526 of 638)	95.3 % (203 of 213)	70.6 % (226 of 320)	92.4 % (97 of 105)
Sidechain	94.5 % (723 of 765)	93.9 % (434 of 462)	95.3 % (285 of 299)	100.0 % (4 of 4)
Aromatic	68.9 % (62 of 90)	68.9 % (31 of 45)	68.9 % (31 of 45)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. Thermosynechococcus KaiA

AMARMSPADK RKLLDELRSI YRTIVLEYFN TDAKVNERID EFVSKAFFAD ISVSQVLEIH VELMDTFSKQ LKLEGRSEDI LLDYRLTLID VIAHLCEMYR RSIPREV

Show sample condition

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.4 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1Q6A, Strand ID: A, B Detail

Release date

2004-02-12

Citation

Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein
Vakonakis, I., LiWang, A.C.
J. Biomol. NMR (2004), 28, 403-404, PubMed 14872133 , DOI 10.1023/B:JNMR.0000015373.13794.c7 , Abstract

Related entities 1. KaiA C-terminal domain, : 1 : 3 : 4 : 13 entities Detail

Interaction partners 1. KaiA C-terminal domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 15N HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

2 13C HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

3 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

4 CBCANH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

5 HBHA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

6 C(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

7 H(CCO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

8 H(C)CH-COSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

9 4D 13C-13C edited NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

Sample #1

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Thermosynechococcus KaiA	[U-99% 13C; U-99% 15N]	1.2 mM
2	Na2HPO4		20.0 mM
3	NaCl		20.0 mM

Sample #2

Temperature 323 (±0.5) K, pH 7.0 (±0.1)

#	Name	Concentration
4	Thermosynechococcus KaiA	1.2 mM
5	Na2HPO4	20.0 mM
6	NaCl	20.0 mM

nullKeywords assignment, circadian clock, KaiA, Thermosynechococcus elongatus

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Found 1 Document (1.662 seconds)

BMRB: 5824

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. KaiA C-terminal domain, : 1 : 3 : 4 : 13 entities Detail

Interaction partners 1. KaiA C-terminal domain, : 1 interactors Detail

Experiments performed 9 experiments Detail

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