BMRB: 5833

1H, 13C, and 15N Chemical Shift Assignments for Mob1

Authors

Ponchon, L., Dumas, C., Fesquet, D., Padilla, A.

Assembly

mob1

Entity

1. mob1 (polymer, Thiol state: not reported), 202 monomers, 23274.25 Da Detail

MGSSHHHHHH SSGLVPRGSA TLGSGNLRQA VMLPEGEDLN EWIAVNTVDF FNQINMLYGT ITEFCTESTC SVMSAGPRYE YHWADGTNIK KPIKCSAPKY IDYLMTWVQD QLDDETLFPS KIGVPFPKNF MSVAKTILKR LFRVYAHIYH QHFDAVMQLQ EEAHLNTSFK HFIFFVQEFN LIDRRELAPL QELIEKLGSK DR

Formula weight

23274.25 Da

Source organism

Xenopus laevis

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 72.0 %, Completeness (bb): 81.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.0 % (1745 of 2423)	73.9 % (928 of 1256)	68.8 % (656 of 953)	75.2 % (161 of 214)
Backbone	81.5 % (973 of 1194)	80.8 % (328 of 406)	81.8 % (487 of 595)	81.9 % (158 of 193)
Sidechain	65.7 % (933 of 1420)	70.6 % (600 of 850)	60.1 % (330 of 549)	14.3 % (3 of 21)
Aromatic	41.9 % (113 of 270)	75.6 % (102 of 135)	8.3 % (11 of 132)	0.0 % (0 of 3)
Methyl	93.8 % (195 of 208)	93.3 % (97 of 104)	94.2 % (98 of 104)

1. xenopus laevis mob1

MGSSHHHHHH SSGLVPRGSA TLGSGNLRQA VMLPEGEDLN EWIAVNTVDF FNQINMLYGT ITEFCTESTC SVMSAGPRYE YHWADGTNIK KPIKCSAPKY IDYLMTWVQD QLDDETLFPS KIGVPFPKNF MSVAKTILKR LFRVYAHIYH QHFDAVMQLQ EEAHLNTSFK HFIFFVQEFN LIDRRELAPL QELIEKLGSK DR

Show sample condition

Sample

Temperature 300 (±3) K, pH 8.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	xenopus laevis mob1	[U-98% 15N]		0.0 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1R3B, Strand ID: A Detail

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Release date

2004-02-09

Citation

Resonance assignments and topology of the 15N, 13C labelled 23 kDa core domain of xenopus Mob1
Ponchon, L., Dumas, C., Fesquet, D., Padilla, A.
J. Biomol. NMR (2004), 28, 299-300, PubMed 14752264 , DOI 10.1023/B:JNMR.0000013691.50297.11 , Abstract

Related entities 1. mob1, : 1 : 118 entities Detail

Interaction partners 1. mob1, : 1 interactors Detail