BMRB: 5848

Solution structure of S8-SP11

Authors

Mishima, M., Takayama, S., Sasaki, K., Jee, J., Kojima, C., Isogai, A., Shirakawa, M.

Assembly

S-locus pollen protein

Entity

1. S-locus pollen protein (polymer, Thiol state: all disulfide bound), 50 monomers, 5724.665 Da Detail

NLMKRCTRGF RKLGKCTTLE EEKCKTLYPR GQCTCSDSKM NTHSCDCKSC

Formula weight

5724.665 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS50:SG
2	disulfide	sing	1:CYS16:SG	1:CYS35:SG
3	disulfide	sing	1:CYS24:SG	1:CYS45:SG
4	disulfide	sing	1:CYS33:SG	1:CYS47:SG

Source organism

Brassica rapa

Exptl. method

NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 86.9 %, Completeness (bb): 93.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.9 % (265 of 305)	86.9 % (265 of 305)
Backbone	93.1 % (95 of 102)	93.1 % (95 of 102)
Sidechain	83.7 % (170 of 203)	83.7 % (170 of 203)
Aromatic	72.7 % (8 of 11)	72.7 % (8 of 11)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. S-locus pollen protein

NLMKRCTRGF RKLGKCTTLE EEKCKTLYPR GQCTCSDSKM NTHSCDCKSC

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	S-locus pollen protein			0.5 mM
2	phosphate buffer			50 mM
3	KCl			50 mM
4	H2O			95 %
5	D2O			5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	S-locus pollen protein			0.5 mM
7	phosphate buffer			50 mM
8	KCl			50 mM
9	D2O			99.8 %

Hide sample codition

Protein Blocks Logo

Calculated from 30 models in PDB: 1UGL, Strand ID: A Detail

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Release date

2003-10-05

Citation

Structure of the male determinant factor for Brassica self-incompatibility
Mishima, M., Takayama, S., Sasaki, K., Jee, J.G., Kojima, C., Isogai, A., Shirakawa, M.
J. Biol. Chem. (2003), 278, 36389-36395, PubMed 12835321 , DOI 10.1074/jbc.M305305200 , Abstract

Related entities 1. S-locus pollen protein, : 1 : 1 entities Detail

Experiments performed 3 experiments Detail