BMRB: 5849

Solution structure of the PCI domain

Authors

Suzuki, S., Hatanaka, H., Kigawa, T., Shirouzu, M., Hayashizaki, Y., Yokoyama, S.

Assembly

COP9 complex subunit 4

Entity

1. COP9 complex subunit 4 (polymer, Thiol state: not present), 84 monomers, 8793.690 Da Detail

GSSGSSGGSS ILDRAVIEHN LLSASKLYNN ITFEELGALL EIPAAKAEKI ASQMITEGRM NGFIDQIDGI VHFETREASG PSSG

Formula weight

8793.69 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 94.5 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (859 of 909)	91.2 % (427 of 468)	98.0 % (346 of 353)	97.7 % (86 of 88)
Backbone	97.0 % (485 of 500)	96.6 % (170 of 176)	97.1 % (235 of 242)	97.6 % (80 of 82)
Sidechain	92.5 % (447 of 483)	88.0 % (257 of 292)	99.5 % (184 of 185)	100.0 % (6 of 6)
Aromatic	87.0 % (40 of 46)	73.9 % (17 of 23)	100.0 % (23 of 23)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. COP9 complex subunit 4

GSSGSSGGSS ILDRAVIEHN LLSASKLYNN ITFEELGALL EIPAAKAEKI ASQMITEGRM NGFIDQIDGI VHFETREASG PSSG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	COP9 complex subunit 4	[U-13C; U-15N]		6.1 mM
2	sodium phosphate buffer			20 mM
3	NaCl			100 mM
4	d-DTT			1 mM
5	dithiothreitol	[U-100% 2H]		1 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	na	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	na	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	na	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	na	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UFM, Strand ID: A Detail

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Release date

2004-09-13

Citation

Solution structure of the PCI domain
Suzuki, S., Hatanaka, H., Kigawa, T., Shirouzu, M., Hayashizaki, Y., Yokoyama, S.

Related entities 1. COP9 complex subunit 4, : 1 : 67 entities Detail

Interaction partners 1. COP9 complex subunit 4, : 6 interactors Detail

Experiments performed 2 experiments Detail