BMRB: 5850

Kinetic and Structural Studies of the Low Moleuclar Weight Protein Tyrosine Phosphatase from Tritrichomonas foetus

Authors

Thomas, C.L., Hallenga, K., Stauffacher, C.V., Van Etten, R.L.

Assembly

protein tyrosine phosphatase (E.C.3.1.3.48)

Entity

1. protein tyrosine phosphatase (E.C.3.1.3.48) (polymer, Thiol state: all free), 146 monomers, 15820.99 Da Detail

AAEKKAVLFV CLGNICRSPA CEGICRDMVG DKLIIDSAAT SGFHVGQSPD TRSQKVCKSN GVDISKQRAR QITKADFSKF DVIAALDQSI LSDINSMKPS NCRAKVVLFN PPNGVDDPYY SSDGFPTMFA SISKEMKPFL TEHGLI

Formula weight

15820.99 Da

Source organism

Tritrichomonas foetus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.2 %, Completeness (bb): 82.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.2 % (1343 of 1653)	86.9 % (747 of 860)	69.7 % (449 of 644)	98.7 % (147 of 149)
Backbone	82.7 % (711 of 860)	98.6 % (289 of 293)	66.7 % (286 of 429)	98.6 % (136 of 138)
Sidechain	82.2 % (764 of 930)	80.8 % (458 of 567)	83.8 % (295 of 352)	100.0 % (11 of 11)
Aromatic	43.3 % (45 of 104)	51.9 % (27 of 52)	34.6 % (18 of 52)
Methyl	98.7 % (148 of 150)	98.7 % (74 of 75)	98.7 % (74 of 75)

1. protein tyrosine phosphatase (E.C.3.1.3.48)

AAEKKAVLFV CLGNICRSPA CEGICRDMVG DKLIIDSAAT SGFHVGQSPD TRSQKVCKSN GVDISKQRAR QITKADFSKF DVIAALDQSI LSDINSMKPS NCRAKVVLFN PPNGVDDPYY SSDGFPTMFA SISKEMKPFL TEHGLI

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.2

#	Name	Isotope labeling	Type	Concentration
1	protein tyrosine phosphatase (E.C.3.1.3.48)	[U-15N; U-13C]		1.0 ~ 2.0 mM
2	NaCl			130 mM
3	NaH2PO4			20 mM
4	DSS			1 mM
5	H20			90 %
6	D2O			10 %

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Protein Blocks Logo

Calculated from 21 models in PDB: 1P8A, Strand ID: A Detail

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Release date

2004-05-14

Citation 1

Solution structure of the low-molecular-weight protein tyrosine phosphatase from Tritrichomonas foetus reveals a flexible phosphate binding loop
Gustafson, C.L.T., Stauffacher, C.V., Hallenga, K., Van Etten, R.L.
Protein Sci. (2005), 14, 2515-2525, PubMed 16195543 , DOI 10.1110/ps.051618805 , Abstract

Show more 1 citaion

Citation 2

Kinetic and spectroscopic studies of Tritrichomonas foetus low-molecular weight phosphotyrosyl phosphatase. Hydrogen bond networks and electrostatic effects
Thomas, C.L., McKinnon, E., Granger, B.L., Harms, E., Van Etten, R.L.
Biochemistry (2002), 41, 15601-15609, PubMed 12501188 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. protein tyrosine phosphatase (E.C.3.1.3.48), : 1 : 103 entities Detail

Experiments performed 3 experiments Detail