BMRB: 5851

Solution Stucture of the Third Zinc Finger from BKLF

Authors

Simpson, R.J.Y., Cram, E.D., Czolij, R., Matthews, J.M., Crossley, M., Mackay, J.P.

Assembly

Kruppel-like factor 3

Entity

1. Kruppel-like factor 3 (polymer, Thiol state: all other bound), 37 monomers, 4197.745 Da Detail

GSTRGSTGIK PFQCPDCDRS FSRSDHLALH RKRHMLV

2. ZN (non-polymer), 65.409 Da

Total weight

4263.1543 Da

Max. entity weight

4197.745 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS14:SG	2:ZN1:ZN
2	na	sing	1:CYS17:SG	2:ZN1:ZN
3	na	sing	1:HIS30:NE2	2:ZN1:ZN
4	na	sing	1:HIS34:NE2	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 84.6 %, Completeness (bb): 91.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	84.6 % (219 of 259)	83.4 % (186 of 223)	91.7 % (33 of 36)
Backbone	91.8 % (101 of 110)	90.7 % (68 of 75)	94.3 % (33 of 35)
Sidechain	79.2 % (118 of 149)	79.7 % (118 of 148)	0.0 % (0 of 1)
Aromatic	43.8 % (7 of 16)	43.8 % (7 of 16)
Methyl	92.3 % (12 of 13)	92.3 % (12 of 13)

1. Kruppel-like factor 3

GSTRGSTGIK PFQCPDCDRS FSRSDHLALH RKRHMLV

Show sample condition

Sample

Pressure 1 atm, Temperature 280 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Kruppel-like factor 3			3 mM
2	TCEP			4.5 mM
3	ZnSO4			4.5 mM
4	H2O			90 %
5	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1P7A, Strand ID: A Detail

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Coupling constant

28 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 280 K, pH 5.5 Detail

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Pressure 1 atm, Temperature 280 K, pH 5.5

List #1 coupling_constants_set_1, Field strength (¹H) 600 MHz

Release date

2003-09-11

Citation

CCHX zinc finger derivatives retain the ability to bind Zn(II) and mediate protein-DNA interactions
Simpson, R.J.Y., Cram, E.D., Czolij, R., Matthews, J.M., Crossley, M., Mackay, J.P.
J. Biol. Chem. (2003), 278, 28011-28018, PubMed 12736264 , DOI 10.1074/jbc.M211146200 , Abstract

Related entities 1. Kruppel-like factor 3, : 1 : 6 : 598 entities Detail

Interaction partners 1. Kruppel-like factor 3, : 9 interactors Detail

Experiments performed 4 experiments Detail