BMRB: 5861

Backbone resonance assignments of GTPase domain of E.coli MnmE

Authors

Monleon, D., Yim, L., Martinez-Vicente, M., Armengod, M.EUGENIA., Celda, B.

Assembly

gtpase domain MnmE

Entity

1. gtpase domain MnmE (polymer, Thiol state: not present), 172 monomers, 18697.13 Da Detail

GSLLREGMKV VIAGRPNAGK SSLLNALAGR EAAIVTDIAG TTRDVLREHI HIDGMPLHII DTAGLREASD EVERIGIERA WQEIEQADRV LFMVDGTTTD AVDPAEIWPE FIARLPAKLP ITVVRNKADI TGETLGMSEV NGHALIRLSA RTGEGVDVLR NHLKQSMGIH RD

Formula weight

18697.13 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

NMR restraints were obtained from the backbone assignment (TALOS analysis for dihedral angle constraints) and from the 15N-edited 3D NOESY. Additional structural information was obtained from the homologous GTPAse protein structures.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 52.5 %, Completeness (bb): 94.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	52.5 % (1003 of 1910)	36.8 % (362 of 985)	64.1 % (480 of 749)	91.5 % (161 of 176)
Backbone	94.0 % (959 of 1020)	93.5 % (331 of 354)	93.4 % (467 of 500)	97.0 % (161 of 166)
Sidechain	17.6 % (184 of 1046)	4.9 % (31 of 631)	37.8 % (153 of 405)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 34)	0.0 % (0 of 32)	0.0 % (0 of 2)
Methyl	15.5 % (37 of 238)	10.1 % (12 of 119)	21.0 % (25 of 119)

1. gtpase domain mnme

GSLLREGMKV VIAGRPNAGK SSLLNALAGR EAAIVTDIAG TTRDVLREHI HIDGMPLHII DTAGLREASD EVERIGIERA WQEIEQADRV LFMVDGTTTD AVDPAEIWPE FIARLPAKLP ITVVRNKADI TGETLGMSEV NGHALIRLSA RTGEGVDVLR NHLKQSMGIH RD

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gtpase domain mnme	[U-95% 13C; U-95% 15N]		0.2 ~ 0.4 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1RFL, Strand ID: A Detail

---

Release date

2004-02-10

Citation

Backbone 1H, 13C and 15N resonance assignments for the 18.7 kDa GTPase domain of Escherichia coli MnmE protein
Monleon, D., Yim, L., Martinez-Vicente, M., Armengod, M.EUGENIA., Celda, B.
J. Biomol. NMR (2004), 28, 307-308, PubMed 14752268 , DOI 10.1023/B:JNMR.0000013693.80516.57 , Abstract

Related entities 1. gtpase domain MnmE, : 1 : 10 : 189 entities Detail

Interaction partners 1. gtpase domain MnmE, : 8 interactors Detail

Experiments performed 6 experiments Detail