BMRB: 5865

Solution Structure of Bmkk 2, A Novel Potassium Channel Blocker from Scorpion Buthus martensi Karsch

Authors

Wu, H., Zhang, N., Wang, Y., Zhang, Q., Ou, L., Li, M., Hu, G.

Assembly

K+ toxin-like peptide

Entity

1. K+ toxin-like peptide (polymer, Thiol state: all disulfide bound), 31 monomers, 3248.735 Da Detail

TPFAIKCATD ADCSRKCPGN PPCRNGFCAC T

Formula weight

3248.735 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS23:SG
2	disulfide	sing	1:CYS13:SG	1:CYS28:SG
3	disulfide	sing	1:CYS17:SG	1:CYS30:SG

Source organism

Mesobuthus martensii

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.8 %, Completeness (bb): 98.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.8 % (167 of 169)	98.8 % (167 of 169)
Backbone	98.3 % (59 of 60)	98.3 % (59 of 60)
Sidechain	99.1 % (108 of 109)	99.1 % (108 of 109)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. K+ toxin-like peptide

TPFAIKCATD ADCSRKCPGN PPCRNGFCAC T

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 3.0

#	Name	Isotope labeling	Type	Concentration
1	K+ toxin-like peptide			2.7 mM
2	H2O			90 %
3	D2O			10 %

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Calculated from 15 models in PDB: 1PVZ, Strand ID: A Detail

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Release date

2003-07-13

Citation

Solution structure of BmKK2, a new potassium channel blocker from the venom of chinese scorpion Buthus martensi Karsch
Wu, G., Zhang, N., Li, M., Chen, X., Wang, Y., Hu, G., Wu, H.
Proteins (2004), 55, 835-845, PubMed 15146482 , DOI 10.1002/prot.20117 , Abstract

Related entities 1. K+ toxin-like peptide, : 1 : 1 : 1 : 1 : 17 entities Detail

Experiments performed 3 experiments Detail