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Found 1 Document (1.782 seconds)

BMRB: 5866

1PPX

Solution Structure and NH Exchange Studies of the MutT Pyrophosphohydrolase Complexed with Mg(2+) and 8-oxo-dGMP, a Tightly-bound Product

Authors

Massiah, M.A., Saraswat, V., Azurmendi, H.F., Mildvan, A.S.

Assembly

Mutator mutT protein (E.C.3.6.1.-)

Entity

1. Mutator mutT protein (polymer, Thiol state: not present), 129 monomers, 14926.87 Da Detail

MKKLQIAVGI IRNENNEIFI TRRAADAHMA NKLEFPGGKI EMGETPEQAV VRELQEEVGI TPQHFSLFEK LEYEFPDRHI TLWFWLVERW EGEPWGKEGQ PGEWMSLVGL NADDFPPANE PVIAKLKRL

2. 8OG (non-polymer), 363.221 Da
3. MG (non-polymer), 24.305 Da

Total weight

15314.3955 Da

Max. entity weight

14926.87 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 71.6 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.6 % (1129 of 1577)	83.5 % (692 of 829)	51.3 % (314 of 612)	90.4 % (123 of 136)
Backbone	80.0 % (605 of 756)	96.9 % (251 of 259)	63.1 % (238 of 377)	96.7 % (116 of 120)
Sidechain	66.8 % (628 of 940)	77.4 % (441 of 570)	50.8 % (180 of 354)	43.8 % (7 of 16)
Aromatic	38.7 % (58 of 150)	65.3 % (49 of 75)	11.4 % (8 of 70)	20.0 % (1 of 5)
Methyl	91.8 % (123 of 134)	98.5 % (66 of 67)	85.1 % (57 of 67)

1. Mutator mutT protein

MKKLQIAVGI IRNENNEIFI TRRAADAHMA NKLEFPGGKI EMGETPEQAV VRELQEEVGI TPQHFSLFEK LEYEFPDRHI TLWFWLVERW EGEPWGKEGQ PGEWMSLVGL NADDFPPANE PVIAKLKRL

Show sample condition

Sample

Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1PPX, Strand ID: A Detail

Release date

2003-10-05

Citation

Solution structure and NH exchange studies of the MutT pyrophosphohydrolase complexed with Mg(2+) and 8-oxo-dGMP, a tightly bound product
Massiah, M.A., Saraswat, V., Azurmendi, H.F., Mildvan, A.S.
Biochemistry (2003), 42, 10140-10154, PubMed 12939141 , DOI 10.1021/bi030105p , Abstract

Related entities 1. Mutator mutT protein, : 1 : 24 : 76 entities Detail

Interaction partners 1. Mutator mutT protein, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

2 2D NOESY

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

3 2D TOCSY

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

4 3D 13C-separated NOESY

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

5 15N-1H IPAP-HSQC

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

6 12C/13C-filtered-NOESY-HSQC

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

7 HNCA

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

8 NHCACB

Instrument

Varian UNITYplus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 295 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Mutator mutT protein			1 mM
2	8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE			1.3 mM
3	MgCl2			15 mM
4	d11-Tris-HCl			4 mM
5	NaCl			21 mM
6	NaN3			0.34 mM
7	H2O			90 %
8	D2O			10 %

nullKeywords MUTATOR PROTEIN, NUCLEOSIDE TRIPHOSPHATE PYROPHOSPHOHYDROLASE, MUTT PYROPHOSPHOHYDROLASE-METAL-PRODUCT COMPLEX