BMRB: 5867

Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)

Authors

Suzuki, S., Hatanaka, H., Koshiba, S., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

INTERSECTIN 2

Entity

1. INTERSECTIN 2 (polymer, Thiol state: not present), 69 monomers, 7264.672 Da Detail

GSSGSSGGEE YIALYPYSSV EPGDLTFTEG EEILVTQKDG EWWTGSIGDR SGIFPSNYVK PKDSGPSSG

Formula weight

7264.672 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 92.9 %, Completeness (bb): 94.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (689 of 742)	88.8 % (340 of 383)	97.9 % (285 of 291)	94.1 % (64 of 68)
Backbone	94.8 % (383 of 404)	92.4 % (134 of 145)	96.9 % (189 of 195)	93.8 % (60 of 64)
Sidechain	91.9 % (363 of 395)	86.6 % (206 of 238)	100.0 % (153 of 153)	100.0 % (4 of 4)
Aromatic	84.2 % (64 of 76)	68.4 % (26 of 38)	100.0 % (36 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (25 of 25)

1. INTERSECTIN 2

GSSGSSGGEE YIALYPYSSV EPGDLTFTEG EEILVTQKDG EWWTGSIGDR SGIFPSNYVK PKDSGPSSG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	INTERSECTIN 2	[U-13C; U-15N]		6.6 mM
2	phosphate buffer Na			20 mM
3	NaCl			100 mM
4	NaN3			0.02 %
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: 2.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	water	na	0.0 ppm	external	indirect	0.0
13C	water	na	0.0 ppm	external	indirect	0.0
1H	water	na	4.773 ppm	internal	direct	0.0

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LACS Plot; CB

Referencing offset: 2.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	water	na	0.0 ppm	external	indirect	0.0
13C	water	na	0.0 ppm	external	indirect	0.0
1H	water	na	4.773 ppm	internal	direct	0.0

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	water	na	0.0 ppm	external	indirect	0.0
13C	water	na	0.0 ppm	external	indirect	0.0
1H	water	na	4.773 ppm	internal	direct	0.0

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LACS Plot; CO

Referencing offset: 2.85 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	water	na	0.0 ppm	external	indirect	0.0
13C	water	na	0.0 ppm	external	indirect	0.0
1H	water	na	4.773 ppm	internal	direct	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UHF, Strand ID: A Detail

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Release date

2006-01-10

Citation

Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)
Suzuki, S., Hatanaka, H., Koshiba, S., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Related entities 1. INTERSECTIN 2, : 1 : 173 entities Detail

Interaction partners 1. INTERSECTIN 2, : 35 interactors Detail

Experiments performed 2 experiments Detail