BMRB: 5868

Solution structure of XPC binding domain of hHR23B

Authors

Kim, B.K., Ryu, K., Kim, H.J., Choi, B.

Assembly

UV excision repair protein RAD23 homolog B

Entity

1. UV excision repair protein RAD23 homolog B (polymer, Thiol state: not present), 72 monomers, 8139.094 Da Detail

GSHMPLEFLR NQPQFQQMRQ IIQQNPSLLP ALLQQIGREN PQLLQQISQH QEHFIQMLNE PVQEAGGQGG GG

Formula weight

8139.094 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Automatic NOE assignment, distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 85.8 %, Completeness (bb): 94.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.8 % (752 of 876)	84.9 % (399 of 470)	85.9 % (274 of 319)	90.8 % (79 of 87)
Backbone	94.8 % (398 of 420)	96.6 % (141 of 146)	92.3 % (192 of 208)	98.5 % (65 of 66)
Sidechain	80.0 % (416 of 520)	79.6 % (258 of 324)	82.3 % (144 of 175)	66.7 % (14 of 21)
Aromatic	0.0 % (0 of 42)	0.0 % (0 of 21)	0.0 % (0 of 21)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. RAD23 homolog B

GSHMPLEFLR NQPQFQQMRQ IIQQNPSLLP ALLQQIGREN PQLLQQISQH QEHFIQMLNE PVQEAGGQGG GG

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	RAD23 homolog B	[U-15N; U-13C]		1 mM
2	phosphate buffer NA			40 mM
3	CHAPS NA			10 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; CO

Referencing offset: -0.39 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1PVE, Strand ID: A Detail

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Release date

2005-05-18

Citation

Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B
Kim, B.K., Ryu, K., Kim, H.J., Cho, S.J., Choi, B.
FEBS J. (2005), 272, 2467-2476, PubMed 15885096 , DOI 10.1111/j.1742-4658.2005.04667.x , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. UV excision repair protein RAD23 homolog B, : 1 : 2 : 44 entities Detail

Interaction partners 1. UV excision repair protein RAD23 homolog B, : 31 interactors Detail

Experiments performed 3 experiments Detail