BMRB: 5874

Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97

Authors

Yuan, X., Simpson, P., Kondo, H., Mckeown, C., Dreveny, I., Shaw, A., Zhang, X., Freemont, P.S., Matthews, S.J.

Assembly

p47 monomer

Entity

1. p47 monomer (polymer, Thiol state: not present), 174 monomers, 19052.42 Da Detail

MAEERQDALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEEEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG ERRR

Formula weight

19052.42 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.9 %, Completeness: 61.4 %, Completeness (bb): 73.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	61.4 % (1176 of 1914)	68.9 % (690 of 1001)	46.3 % (342 of 738)	82.3 % (144 of 175)
Backbone	73.9 % (758 of 1026)	84.3 % (301 of 357)	63.1 % (318 of 504)	84.2 % (139 of 165)
Sidechain	51.4 % (537 of 1044)	60.4 % (389 of 644)	36.7 % (143 of 390)	50.0 % (5 of 10)
Aromatic	22.0 % (29 of 132)	37.9 % (25 of 66)	4.6 % (3 of 65)	100.0 % (1 of 1)
Methyl	60.8 % (90 of 148)	90.5 % (67 of 74)	31.1 % (23 of 74)

1. p47

MAEERQDALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEEEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG ERRR

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	p47	[U-13C; U-15N]		0.5 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.57 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.57 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Release date

2003-12-04

Citation

Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97
Yuan, X., Simpson, P., Kondo, H., Mckeown, C., Dreveny, I., Shaw, A., Zhang, X., Freemont, P.S., Matthews, S.J.
J. Biomol. NMR (2004), 28, 309-310, PubMed 14752269 , DOI 10.1023/B:JNMR.0000013689.02494.07 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. p47 monomer, : 2 : 2 : 18 entities Detail

Interaction partners 1. p47 monomer, : 12 interactors Detail

Experiments performed 8 experiments Detail