1H, 13C, and 15N Chemical Shift Assignments of the dimeric mutant of GB1
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.9 % (538 of 634) | 91.6 % (295 of 322) | 72.8 % (182 of 250) | 98.4 % (61 of 62) |
Backbone | 84.8 % (285 of 336) | 99.1 % (115 of 116) | 70.1 % (115 of 164) | 98.2 % (55 of 56) |
Sidechain | 87.1 % (305 of 350) | 87.4 % (180 of 206) | 86.2 % (119 of 138) | 100.0 % (6 of 6) |
Aromatic | 48.3 % (28 of 58) | 62.1 % (18 of 29) | 32.1 % (9 of 28) | 100.0 % (1 of 1) |
Methyl | 100.0 % (66 of 66) | 100.0 % (33 of 33) | 100.0 % (33 of 33) |
1. Immunoglobulin Binding domain 1 of Protein G
MQYKVILNGK TLKGETTTEA VDAATAEKVV KQFFNDNGVD GEWTYDDATK TFTVTETemperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Immunoglobulin Binding domain 1 of Protein G | [U-95% 13C; U-95% 15N] | 1.7 mM |