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BMRB: 5875

1Q1O

1H, 13C, and 15N Chemical Shift Assignments of the dimeric mutant of GB1

Authors

Byeon, I.L., Louis, J.M., Gronenborn, A.M.

Assembly

The B1 Domain of Streptococcal Protein G

Entity

1. The B1 Domain of Streptococcal Protein G (polymer, Thiol state: not present), 56 monomers, 6220.783 × 2 Da Detail

MQYKVILNGK TLKGETTTEA VDAATAEKVV KQFFNDNGVD GEWTYDDATK TFTVTE

Total weight

12441.566 Da

Max. entity weight

6220.783 Da

Source organism

Streptococcus sp.

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.9 %, Completeness (bb): 84.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (538 of 634)	91.6 % (295 of 322)	72.8 % (182 of 250)	98.4 % (61 of 62)
Backbone	84.8 % (285 of 336)	99.1 % (115 of 116)	70.1 % (115 of 164)	98.2 % (55 of 56)
Sidechain	87.1 % (305 of 350)	87.4 % (180 of 206)	86.2 % (119 of 138)	100.0 % (6 of 6)
Aromatic	48.3 % (28 of 58)	62.1 % (18 of 29)	32.1 % (9 of 28)	100.0 % (1 of 1)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. Immunoglobulin Binding domain 1 of Protein G

MQYKVILNGK TLKGETTTEA VDAATAEKVV KQFFNDNGVD GEWTYDDATK TFTVTE

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Release date

2003-12-04

Citation

A protein contortionist: core mutations of GB1 that induce dimerization and domain swapping
Byeon, I.L., Louis, J.M., Gronenborn, A.M.
J. Mol. Biol. (2003), 333, 141-152, PubMed 14516749 , Abstract

Related entities 1. The B1 Domain of Streptococcal Protein G, : 1 : 2 : 104 entities Detail

Interaction partners 1. The B1 Domain of Streptococcal Protein G, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

2 2D 1H-1H TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

3 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

4 2D 1H-13C HSQC/HMQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

5 3D HNCO

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

6 3D HNCACB

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

7 3D CBCA(CO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

8 3D H(CCO)NH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

9 3D C(CCO)NH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

10 3D HNHB

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

11 3D HACAHB-COSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

12 2D 13C'-{13CG} spin-echo difference

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

13 2D 15N-{13C'} spin-echo difference

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

14 2D 13C'-{13CG} spin-echo difference arom

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

15 2D 15N-{13C'} spin-echo difference arom

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

16 2D [13C-13C] long-range correlation experiment

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

17 3D 15N-edited TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

18 3D 15N-edited NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Immunoglobulin Binding domain 1 of Protein G	[U-95% 13C; U-95% 15N]		1.7 mM

nullKeywords core mutants, domain-swapping, GB1, NMR structure, oligomerization