BMRB: 5876

Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97

Authors

Yuan, X., Simpson, P., Kondo, H., Mckeown, C., Dreveny, I., Shaw, A., Zhang, X., Freemont, P.S., Matthews, S.J.

Assembly

p47 monomer

Entity

1. p47 monomer (polymer, Thiol state: not present), 200 monomers, 22242.70 Da Detail

ERRRHSGQDV HVVLKLWKTG FSLDNGDLRS YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF KAFTGEGQKL GSTAPQVLNT SSPAQQAENE AKASSSILIN EAEPTTNIQI RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFVLM TTFPNKELAD ENQTLKEANL LNAVIVQRLT

Formula weight

22242.7 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 78.5 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.5 % (1808 of 2304)	84.2 % (1013 of 1203)	69.3 % (613 of 884)	83.9 % (182 of 217)
Backbone	94.2 % (1115 of 1184)	93.8 % (379 of 404)	95.4 % (561 of 588)	91.1 % (175 of 192)
Sidechain	66.7 % (872 of 1308)	79.3 % (634 of 799)	47.7 % (231 of 484)	28.0 % (7 of 25)
Aromatic	38.8 % (52 of 134)	70.1 % (47 of 67)	6.1 % (4 of 66)	100.0 % (1 of 1)
Methyl	66.1 % (148 of 224)	96.4 % (108 of 112)	35.7 % (40 of 112)

1. p47

ERRRHSGQDV HVVLKLWKTG FSLDNGDLRS YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF KAFTGEGQKL GSTAPQVLNT SSPAQQAENE AKASSSILIN EAEPTTNIQI RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFVLM TTFPNKELAD ENQTLKEANL LNAVIVQRLT

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	p47	[U-13C; U-15N]		0.5 mM

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LACS Plot; CA

Referencing offset: -0.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 1.3 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1JRU, Strand ID: A Detail

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Release date

2003-12-04

Citation

Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97
Yuan, X., Simpson, P., Kondo, H., Mckeown, C., Dreveny, I., Zhang, X., Freemont, P.S., Matthews, S.J.
J. Biomol. NMR (2004), 28, 309-310, PubMed 14752269 , DOI 10.1023/B:JNMR.0000013689.02494.07 , Abstract

Related entities 1. p47 monomer, : 4 : 56 entities Detail

Interaction partners 1. p47 monomer, : 14 interactors Detail

Experiments performed 8 experiments Detail