BMRB: 5880

Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22

Authors

Suzuki, S., Hatanaka, H., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Hayashizaki, Y., Yokoyama, S.

Assembly

product of RIKEN cDNA 3110009E22

Entity

1. product of RIKEN cDNA 3110009E22 (polymer, Thiol state: not present), 105 monomers, 11565.70 Da Detail

GSSGSSGTPL SLTLDHWSEI RSRAHNLSVE IKKGPWRTFC ASEWPTFDVG WPPEGTFDLT VIFEVKAIVF QDGPGSHPDQ QPYITVWQDL VQNSPPWIKS GPSSG

Formula weight

11565.7 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dyanamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (1123 of 1211)	91.1 % (571 of 627)	93.9 % (448 of 477)	97.2 % (104 of 107)
Backbone	94.4 % (574 of 608)	96.2 % (201 of 209)	92.5 % (282 of 305)	96.8 % (91 of 94)
Sidechain	92.0 % (642 of 698)	88.5 % (370 of 418)	97.0 % (259 of 267)	100.0 % (13 of 13)
Aromatic	85.9 % (122 of 142)	78.9 % (56 of 71)	92.3 % (60 of 65)	100.0 % (6 of 6)
Methyl	97.9 % (94 of 96)	97.9 % (47 of 48)	97.9 % (47 of 48)

1. RIKEN cDNA 3110009E22

GSSGSSGTPL SLTLDHWSEI RSRAHNLSVE IKKGPWRTFC ASEWPTFDVG WPPEGTFDLT VIFEVKAIVF QDGPGSHPDQ QPYITVWQDL VQNSPPWIKS GPSSG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	RIKEN cDNA 3110009E22	[U-13C; U-15N]		3.2 mM
2	phosphate buffer Na			20 mM
3	NaCl			100 mM
4	d-DTT			1 mM
5	H2O			90 %
6	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	external	indirect	0.0
1H	water	protons	4.773 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	external	indirect	0.0

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	external	indirect	0.0
1H	water	protons	4.773 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	external	indirect	0.0

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	external	indirect	0.0
1H	water	protons	4.773 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	external	indirect	0.0

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LACS Plot; CO

Referencing offset: -0.11 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	0.0 ppm	external	indirect	0.0
1H	water	protons	4.773 ppm	internal	direct	1.0
15N	water	protons	0.0 ppm	external	indirect	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UHU, Strand ID: A Detail

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Release date

2006-01-10

Citation

Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22
Suzuki, S., Hatanaka, H., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Hayashizaki, Y., Yokoyama, S.

Related entities 1. product of RIKEN cDNA 3110009E22, : 1 : 39 entities Detail

Experiments performed 2 experiments Detail