BMRB: 5881

Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA

Authors

Keizer, D.W., West, P.J., Lee, E.F., Olivera, B.M., Bulaj, G., Norton, R.S.

Assembly

SmIIIA

Entity

1. SmIIIA (polymer, Thiol state: all disulfide bound), 22 monomers, 2612.961 Da Detail

XRCCNGRRGC SSRWCRDHSR CC

Formula weight

2612.961 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS15:SG
2	disulfide	sing	1:CYS4:SG	1:CYS21:SG
3	disulfide	sing	1:CYS10:SG	1:CYS22:SG

Source organism

Conus stercusmuscarum

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 95.5 %, Completeness: 94.3 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.3 % (115 of 122)	94.3 % (115 of 122)
Backbone	93.2 % (41 of 44)	93.2 % (41 of 44)
Sidechain	94.9 % (74 of 78)	94.9 % (74 of 78)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)

1. SmIIIA mu-conotoxin

XRCCNGRRGC SSRWCRDHSR CC

Show sample condition

Sample

Temperature 278 (±0.1) K, pH 4.70 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	SmIIIA mu-conotoxin			0.9 mM

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Chem. Shift Complete2

Sequence coverage: 95.5 %, Completeness: 95.9 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.9 % (234 of 244)	95.9 % (234 of 244)
Backbone	96.6 % (85 of 88)	96.6 % (85 of 88)
Sidechain	95.5 % (149 of 156)	95.5 % (149 of 156)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)

1. SmIIIA mu-conotoxin

XRCCNGRRGC SSRWCRDHSR CC

Show sample condition

Sample

Temperature 278 (±0.1) K, pH 3.30 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	SmIIIA mu-conotoxin			0.9 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Q2J, Strand ID: A Detail

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Release date

2003-07-24

Citation

Structural basis for tetrodotoxin-resistant sodium channel binding by mu-conotoxin SmIIIA
Keizer, D.W., West, P.J., Lee, E.F., Yoshikami, D., Olivera, B.M., Bulaj, G., Norton, R.S.
J. Biol. Chem. (2003), 278, 46805-46813, PubMed 12970353 , DOI 10.1074/jbc.M309222200 , Abstract

Related entities 1. SmIIIA, : 1 : 1 : 15 entities Detail

Experiments performed 4 experiments Detail