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BMRB: 5882

2OII

Backbone chemical shift assignments for the C-terminal globular domain of EMILIN-1

Authors

Verdone, G., Colebrooke, S.A., Boyd, J., Viglino, P., Corazza, A., Esposito, G., Campbell, I.D.

Assembly

C1q trimer

Entity

1. C1q trimer (polymer, Thiol state: all free), 162 monomers, 17208.04 × 3 Da Detail

MRGSHHHHHH GSPVPQVAFS AALSLPRSEP GTVPFDRVLL NDGGYYDPET GVFTAPLAGR YLLSAVLTGH RHEKVEAVLS RSNQGVARVD SGGYEPEGLE NKPVAESQPS PGTLGVFSLI LPLQAGDTVC VDLVMGQLAH SEEPLTIFSG ALLYGDPELE HA

Total weight

51624.117 Da

Max. entity weight

17208.04 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 42.8 %, Completeness (bb): 74.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.8 % (757 of 1769)	19.8 % (180 of 908)	62.4 % (440 of 705)	87.8 % (137 of 156)
Backbone	74.0 % (699 of 944)	43.3 % (142 of 328)	91.0 % (426 of 468)	88.5 % (131 of 148)
Sidechain	19.0 % (184 of 969)	6.2 % (36 of 580)	37.5 % (143 of 381)	62.5 % (5 of 8)
Aromatic	0.0 % (0 of 130)	0.0 % (0 of 65)	0.0 % (0 of 65)
Methyl	16.5 % (31 of 188)	12.8 % (12 of 94)	20.2 % (19 of 94)

1. C1q domain of h-EMILIN-1

MRGSHHHHHH GSPVPQVAFS AALSLPRSEP GTVPFDRVLL NDGGYYDPET GVFTAPLAGR YLLSAVLTGH RHEKVEAVLS RSNQGVARVD SGGYEPEGLE NKPVAESQPS PGTLGVFSLI LPLQAGDTVC VDLVMGQLAH SEEPLTIFSG ALLYGDPELE HA

Show sample condition

Sample

Temperature 310.0 (±0.1) K, pH 7.50 (±0.01)

#	Name	Isotope labeling	Concentration
1	C1q domain of h-EMILIN-1	[U-100% 13C; U-100% 15N; 80% 2H]	1.8 mM
2	phosphate buffer		20 mM
3	sodium chloride		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	dioxane	methylene protons	3.75 ppm	internal	direct	1.0
¹³C	dioxane	methylene protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	dioxane	methylene protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.57 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	dioxane	methylene protons	3.75 ppm	internal	direct	1.0
¹³C	dioxane	methylene protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	dioxane	methylene protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.57 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	dioxane	methylene protons	3.75 ppm	internal	direct	1.0
¹³C	dioxane	methylene protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	dioxane	methylene protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 1.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2OII, Strand ID: A, B, C Detail

Release date

2004-04-06

Citation

Sequence-specific backbone NMR assignments for the C-terminal globular domain of EMILIN-1
Verdone, G., Colebrooke, S.A., Boyd, J., Viglino, P., Corazza, A., Doliana, R., Mungiguerra, G., Colombatti, A., Esposito, G., Campbell, I.D.
J. Biomol. NMR (2004), 29, 91-92, PubMed 15017143 , DOI 10.1023/B:JNMR.0000019460.94913.6a , Abstract

Related entities 1. C1q trimer, : 1 : 1 : 168 entities Detail

Interaction partners 1. C1q trimer, : 29 interactors Detail

More details for 29 interactors identified by 14 citations