BMRB: 5892

1H, 13C and 15N NMR Assignments of the Hyperthermophile Sulfolobus Protein Sso10b2 and its Predicted Secondary Structure

Authors

Biyani, K., Kahsai, M., Edmondson, S.P., Shriver, J.W.

Assembly

Sso10b2

Entity

1. Sso10b2 (polymer, Thiol state: not present), 89 monomers, 10238.74 × 2 Da Detail

MTEKLNEIVV RKTKNVEDHV LDVIVLFNQG IDEVILKGTG REISKAVDVY NSLKDRLGDG VQLVNVQTGS EVRDRRRISY ILLRLKRVY

Total weight

20477.48 Da

Max. entity weight

10238.74 Da

Source organism

Saccharolobus solfataricus

Exptl. method

NMR

Refine. method

Simulated annealing using cartesian and torsion angle dynamics.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 91.6 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.6 % (977 of 1067)	89.2 % (497 of 557)	94.9 % (392 of 413)	90.7 % (88 of 97)
Backbone	97.4 % (520 of 534)	96.7 % (178 of 184)	98.1 % (256 of 261)	96.6 % (86 of 89)
Sidechain	87.5 % (539 of 616)	85.5 % (319 of 373)	92.8 % (218 of 235)	25.0 % (2 of 8)
Aromatic	89.5 % (34 of 38)	89.5 % (17 of 19)	89.5 % (17 of 19)
Methyl	94.0 % (126 of 134)	94.0 % (63 of 67)	94.0 % (63 of 67)

1. Sso10b2

MTEKLNEIVV RKTKNVEDHV LDVIVLFNQG IDEVILKGTG REISKAVDVY NSLKDRLGDG VQLVNVQTGS EVRDRRRISY ILLRLKRVY

Show sample condition

Sample

Temperature 303 (±0.2) K, pH 4.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Sso10b2	[U-98% 13C; U-98% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.26 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 11 models in PDB: 2A2Y, Strand ID: A, B Detail

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Release date

2004-07-29

Citation

Solution structure, stability, and nucleic acid binding of the hyperthermophile protein Sso10b2
Biyani, K., Kahsai, M.A., Clark, A.T., Armstrong, T.L., Edmondson, S.P., Shriver, J.W.
Biochemistry (2005), 44, 14217-14230, PubMed 16245938 , DOI 10.1021/bi051266r , Abstract

Related entities 1. Sso10b2, : 1 : 3 : 16 entities Detail

Interaction partners 1. Sso10b2, : 1 interactors Detail