BMRB: 5900

NMR structure of 16th module of Immune Adherence Receptor, Cr1 (Cd35)

Authors

O'Leary, J.M., Bromek, K., Black, G.M., Uhrinova, S., Schmitz, C., Krych, M., Atkinson, J.P., Uhrin, D., Barlow, P.N.

Assembly

complement receptor type 1

Entity

1. complement receptor type 1 (polymer, Thiol state: all disulfide bound), 68 monomers, 7299.243 Da Detail

EAEAKSCKTP PDPVNGMVHV ITDIQVGSRI TYSCTTGHRL IGHSSAECIL SGNTAHWSTK PPICQRIP

Formula weight

7299.243 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS48:SG
2	disulfide	sing	1:CYS34:SG	1:CYS64:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Simulated annealing and molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.1 %, Completeness: 86.9 %, Completeness (bb): 93.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	86.9 % (392 of 451)	85.7 % (329 of 384)	94.0 % (63 of 67)
Backbone	93.4 % (184 of 197)	93.3 % (126 of 135)	93.5 % (58 of 62)
Sidechain	81.9 % (208 of 254)	81.5 % (203 of 249)	100.0 % (5 of 5)
Aromatic	84.2 % (16 of 19)	83.3 % (15 of 18)	100.0 % (1 of 1)
Methyl	91.9 % (34 of 37)	91.9 % (34 of 37)

1. complement receptor type 1

EAEAKSCKTP PDPVNGMVHV ITDIQVGSRI TYSCTTGHRL IGHSSAECIL SGNTAHWSTK PPICQRIP

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	complement receptor type 1	[U-15N]		1 mM
2	phosphate buffer			25 mM
3	D2O			10 %
4	H2O			90 %

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Chem. Shift Complete2

Sequence coverage: 13.2 %, Completeness: 47.0 %, Completeness (bb): 53.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	47.0 % (424 of 902)	46.0 % (353 of 768)	53.0 % (71 of 134)
Backbone	53.0 % (209 of 394)	53.0 % (143 of 270)	53.2 % (66 of 124)
Sidechain	42.3 % (215 of 508)	42.2 % (210 of 498)	50.0 % (5 of 10)
Aromatic	42.1 % (16 of 38)	41.7 % (15 of 36)	50.0 % (1 of 2)
Methyl	50.0 % (37 of 74)	50.0 % (37 of 74)

1. complement receptor type 1

EAEAKSCKTP PDPVNGMVHV ITDIQVGSRI TYSCTTGHRL IGHSSAECIL SGNTAHWSTK PPICQRIP

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	complement receptor type 1	[U-15N]		1 mM
2	phosphate buffer			25 mM
3	D2O			10 %
4	H2O			90 %

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Chem. Shift Complete3

Sequence coverage: 10.3 %, Completeness: 33.1 %, Completeness (bb): 38.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	33.1 % (448 of 1353)	32.2 % (371 of 1152)	38.3 % (77 of 201)
Backbone	38.7 % (229 of 591)	38.8 % (157 of 405)	38.7 % (72 of 186)
Sidechain	28.7 % (219 of 762)	28.6 % (214 of 747)	33.3 % (5 of 15)
Aromatic	28.1 % (16 of 57)	27.8 % (15 of 54)	33.3 % (1 of 3)
Methyl	34.2 % (38 of 111)	34.2 % (38 of 111)

1. complement receptor type 1

EAEAKSCKTP PDPVNGMVHV ITDIQVGSRI TYSCTTGHRL IGHSSAECIL SGNTAHWSTK PPICQRIP

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	complement receptor type 1	[U-15N]		1 mM
2	phosphate buffer			25 mM
3	D2O			10 %
4	H2O			90 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1PPQ, Strand ID: A Detail

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Release date

2003-08-06

Citation

Backbone dynamics of complement control protein (CCP) modules reveals mobility in binding surfaces
O'Leary, J.M., Bromek, K., Black, G.M., Uhrinova, S., Schmitz, C., Wang, X., Krych, M., Atkinson, J.P., Uhrin, D., Barlow, P.N.
Protein Sci. (2004), 13, 1238-1250, PubMed 15096630 , DOI 10.1110/ps.03582704 , Abstract

Related entities 1. complement receptor type 1, : 1 : 1 : 558 entities Detail

Interaction partners 1. complement receptor type 1, : 7 interactors Detail

Experiments performed 6 experiments Detail