BMRBj - BMREntryMaster

Found 1 Document (1.84 seconds)

BMRB: 5901

1PXE

Solution Strucure of a CCHHC Domain of Neural Zinc Finger Factor-1

Authors

Berkovits-Cymet, H.J., Amann, B.T., Berg, J.M.

Assembly

neural zinc finger transcription factor 1

Entity

1. neural zinc finger transcription factor 1 (polymer, Thiol state: all other bound), 63 monomers, 7120.075 Da Detail

MHVKKPYYDP SRTEKRESKC PTPGCDGTGH VTGLYPHHRS LSGCPHKDRV PPEILAMHEN VLK

2. ZN (non-polymer), 65.409 Da

Total weight

7185.4844 Da

Max. entity weight

7120.075 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS20:SG	2:ZN1:ZN
2	na	sing	1:CYS25:SG	2:ZN1:ZN
3	na	sing	1:CYS44:SG	2:ZN1:ZN
4	na	sing	1:HIS38:NE2	2:ZN1:ZN

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 68.3 %, Completeness: 51.7 %, Completeness (bb): 70.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	51.7 % (228 of 441)	48.6 % (187 of 385)	73.2 % (41 of 56)
Backbone	70.8 % (126 of 178)	69.1 % (85 of 123)	74.5 % (41 of 55)
Sidechain	38.8 % (102 of 263)	38.9 % (102 of 262)	0.0 % (0 of 1)
Aromatic	50.0 % (12 of 24)	50.0 % (12 of 24)
Methyl	69.6 % (16 of 23)	69.6 % (16 of 23)

1. neural zinc finger transcription factor 1

MHVKKPYYDP SRTEKRESKC PTPGCDGTGH VTGLYPHHRS LSGCPHKDRV PPEILAMHEN VLK

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.95

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Protein Blocks Logo

Calculated from 21 models in PDB: 1PXE, Strand ID: A Detail

Release date

2004-03-06

Citation

Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding
Berkovits-Cymet, H.J., Amann, B.T., Berg, J.M.
Biochemistry (2004), 43, 898-903, PubMed 14744132 , DOI 10.1021/bi035159d , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P70475 released on 2005-09-27
Title MYT1L_RAT Entity Myelin transcription factor 1-like protein

Related entities 1. neural zinc finger transcription factor 1, : 1 : 2 : 24 entities Detail

Interaction partners 1. neural zinc finger transcription factor 1, : 3 interactors Detail

More details for 3 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 DQF-COSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

2 2D TOCSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

3 2D NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

4 15N HSQC

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

5 15N HSQC LongRange

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

6 3D 15N HSQC-TOCSY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

7 3D 15N HSQC-NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Concentration
1	neural zinc finger transcription factor 1	2.3 mM
2	ZnCl2	2.3 mM
3	deuterated Tris	55 mM

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
4	neural zinc finger transcription factor 1	[U-15N]	2.8 mM
5	ZnCl2		2.8 mM
6	deuterated Tris		36 mM
7	H2O		98 %
8	D2O		2 %

Sample #3

Pressure 1 atm

#	Name	Isotope labeling	Concentration
9	neural zinc finger transcription factor 1	[U-15N]	1.5 mM
10	CdCl2		1.5 mM
11	deuterated Tris		36 mM

nullKeywords CCHHC zinc binding domain, neural zinc finger factor-1, DNA binding domain

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Found 1 Document (1.84 seconds)

BMRB: 5901

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. neural zinc finger transcription factor 1, : 1 : 2 : 24 entities Detail

Interaction partners 1. neural zinc finger transcription factor 1, : 3 interactors Detail

Experiments performed 7 experiments Detail

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