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BMRB: 5902

1H3Z

1H, 13C, and 15N Resonance Assignments of the Hath-Domain of Hepatoma-derived Growth Factor

Authors

Sue, S., Huang, T.

Assembly

Hath domain of hepatoma-derived growth factor (hHDGF)

Entity

1. Hath domain of hepatoma-derived growth factor (hHDGF) (polymer, Thiol state: all free), 110 monomers, 12772.34 Da Detail

MHHHHHHAMA MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY

Formula weight

12772.34 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 88.4 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.4 % (1183 of 1338)	88.5 % (624 of 705)	87.7 % (457 of 521)	91.1 % (102 of 112)
Backbone	90.9 % (587 of 646)	91.0 % (201 of 221)	91.0 % (293 of 322)	90.3 % (93 of 103)
Sidechain	86.6 % (688 of 794)	87.4 % (423 of 484)	85.0 % (256 of 301)	100.0 % (9 of 9)
Aromatic	70.5 % (124 of 176)	70.5 % (62 of 88)	69.8 % (60 of 86)	100.0 % (2 of 2)
Methyl	90.9 % (60 of 66)	90.9 % (30 of 33)	90.9 % (30 of 33)

1. hath domain of hHDGF

MHHHHHHAMA MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Release date

2004-03-06

Citation 1

Sequence specific 1H, 13C and 15N resonance assignments of the hath-domain of human hepatoma-derived growth factor
Sue, S., Chen, J., Huang, T.
J. Biomol. NMR (2004), 29, 95-96, PubMed 15017145 , DOI 10.1023/B:JNMR.0000019467.57276.05 , Abstract

Show more 4 citaions

Citation 2

The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies
Dietz, F., Franken, S., Yoshida, K., Nakamura, H., Kappler, J., Gieselmann, V.
Biochem. J. (2002), 366, 491-500, PubMed 12006088 , DOI 10.1042/BJ20011811 , Abstract

Citation 3

Hepatoma-derived growth factor stimulates cell growth after translocation to the nucleus by nuclear localization signals
Kishima, Y., Yamamoto, H., Izumoto, Y., Yoshida, K., Enomoto, H., Yamamoto, M., Kuroda, T., Ito, H., Yoshizaki, K., Nakamura, H.
J. Biol. Chem. (2002), 277, 10315-10322, PubMed 11751870 , DOI 10.1074/jbc.M111122200 , Abstract

Citation 4

Structural variation in PWWP domains
Slater, L.M., Allen, M.D., Bycroft, M.
J. Mol. Biol. (2003), 330, 571-576, PubMed 12842472 , DOI 10.1016/s0022-2836(03)00470-4 , Abstract

Citation 5

Nuclear targeting is required for hepatoma-derived growth factor-stimulated mitogenesis in vascular smooth muscle cells
Everett, A.D., Stoops, T., McNamara, C.A.
J. Biol. Chem. (2001), 276, 37564-37568, PubMed 11481329 , DOI 10.1074/jbc.M105109200 , Abstract

Hide non-primary 4 citaions

Related entities 1. Hath domain of hepatoma-derived growth factor (hHDGF), : 1 : 9 : 34 entities Detail

Interaction partners 1. Hath domain of hepatoma-derived growth factor (hHDGF), : 13 interactors Detail

More details for 13 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

2 HNCA

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

3 HN(CO)CA

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

4 HNCO

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

5 HN(CA)CO

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

6 CBCANH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

7 CBCA(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

8 HBHA(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

9 1H-15N TOCSY

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

10 H(CC)(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

11 CC(CO)NH

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

12 HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE (600 MHz)

#2 Model Bruker AVANCE (500 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	hath domain of hHDGF	[U-13C; U-15N]	2.0 mM
2	Na2HPO4		100 mM
3	NaCl		150 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	hath domain of hHDGF	[U-13C; U-15N]	1.0 mM
5	Na2HPO4		100 mM
6	NaCl		150 mM
7	D2O		100 %

nullKeywords HDGF, hath-domain, growth factor, PWWP

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Found 1 Document (5.35 seconds)

BMRB: 5902

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Hath domain of hepatoma-derived growth factor (hHDGF), : 1 : 9 : 34 entities Detail

Interaction partners 1. Hath domain of hepatoma-derived growth factor (hHDGF), : 13 interactors Detail

Experiments performed 12 experiments Detail

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