BMRB: 5915

NMR structure of Methionine-Enkephalin in fast tumbling DMPC/DHPC bicelles

Authors

Marcotte, I., Separovic, F., Auger, M., Gagne, S.M.

Assembly

Met-enkephalin 1

Entity

1. Met-enkephalin 1 (polymer, Thiol state: not present), 5 monomers, 573.6619 Da Detail

YGGFM

2. PX4 (non-polymer), 678.940 Da

Total weight

1252.6019 Da

Max. entity weight

678.94 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.3 %, Completeness (bb): 91.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	83.3 % (25 of 30)	83.3 % (25 of 30)
Backbone	91.7 % (11 of 12)	91.7 % (11 of 12)
Sidechain	77.8 % (14 of 18)	77.8 % (14 of 18)
Aromatic	55.6 % (5 of 9)	55.6 % (5 of 9)

1. Met-enkephalin 1

YGGFM

Show sample condition

Sample

Pressure 1 atm, Temperature 295 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	Met-enkephalin 1			5.4 uM
2	Menk DMPC/DHPC 0.5:1 Lipid/peptide: 25:1			10 % w/v
3	H2O			90 %
4	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 80 models in PDB: 1PLW, Strand ID: A Detail

---

Release date

2004-03-06

Citation

A multidimensional 1H NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles
Marcotte, I., Separovic, F., Auger, M., Gagne, S.M.
Biophys. J. (2004), 86, 1587-1600, PubMed 14990485 , DOI 10.1016/S0006-3495(04)74226-5 , Abstract

Experiments performed 2 experiments Detail