BMRB: 5917

Backbone 1HN, 13C, 15N Chemical Shift Assignments for human eIF2alpha

Authors

Ito, T.

Assembly

translation

Entity

1. translation (polymer, Thiol state: all free), 308 monomers, 35685.28 Da Detail

MSCRFYQHKF PEVEDVVMVN VRSIQEMGAY VSLLEYNNIE GMIHLSELSR RRIRSINKLI RIGRNECVKV IRVDKEKGYI DLSKRRVSPE EAIKCEDKFT KSKTVYSILR HVAEVLEYTK DEQLESLFQR TAWVFDDKYK RPGYGAYDAF KHAVSDPSIL DSLDLNEDER EVLINNINRR LTPQAVKIRA DIEVACYGYE GIDAVKEALR AGLNCSTENM PIKINLIAPP RYVMTTTTLE RTEGLSVLSQ AMAVIKEKIE EKRGVFNVQM EPKVVTDTDE TELARQMERL ERENAEVDGD LEHHHHHH

Formula weight

35685.28 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 37.7 %, Completeness (bb): 66.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	37.7 % (1376 of 3651)	24.1 % (460 of 1911)	44.7 % (634 of 1418)	87.6 % (282 of 322)
Backbone	66.6 % (1218 of 1830)	53.2 % (329 of 619)	66.8 % (609 of 912)	93.6 % (280 of 299)
Sidechain	9.3 % (197 of 2117)	10.2 % (132 of 1292)	7.9 % (63 of 802)	8.7 % (2 of 23)
Aromatic	1.8 % (4 of 218)	3.7 % (4 of 109)	0.0 % (0 of 108)	0.0 % (0 of 1)
Methyl	10.4 % (37 of 356)	14.0 % (25 of 178)	6.7 % (12 of 178)

1. eIF2alpha

MSCRFYQHKF PEVEDVVMVN VRSIQEMGAY VSLLEYNNIE GMIHLSELSR RRIRSINKLI RIGRNECVKV IRVDKEKGYI DLSKRRVSPE EAIKCEDKFT KSKTVYSILR HVAEVLEYTK DEQLESLFQR TAWVFDDKYK RPGYGAYDAF KHAVSDPSIL DSLDLNEDER EVLINNINRR LTPQAVKIRA DIEVACYGYE GIDAVKEALR AGLNCSTENM PIKINLIAPP RYVMTTTTLE RTEGLSVLSQ AMAVIKEKIE EKRGVFNVQM EPKVVTDTDE TELARQMERL ERENAEVDGD LEHHHHHH

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	eIF2alpha	[U-2H; U-13C; U-15N]		0.5 ~ 0.7 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1Q8K, Strand ID: A Detail

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Release date

2004-02-12

Citation

Using condon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of Human eIF2a
Ito, T., Wagner, G.
J. Biomol. NMR (2004), 28, 357-367, DOI 10.1023/b:jnmr.0000015405.62261.cb

Related entities 1. translation, : 1 : 104 entities Detail

Interaction partners 1. translation, : 51 interactors Detail