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BMRB: 5919

1MNX

The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts

Authors

Vallurupalli, P., Moore, P.B.

Assembly

Loop E of 5S rRNA from spinach chloroplasts

Entity

1. Loop E of 5S rRNA from spinach chloroplasts (polymer, Thiol state: not present), 42 monomers, 13730.20 Da Detail

GGGUGACGAU ACUGUAGGCG AGAGCCUGCG GAAAAAUAGC CC

Formula weight

13730.2 Da

Source organism

Spinacia oleracea

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 57.9 %, Completeness (bb): 44.4 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	57.9 % (382 of 660)	70.3 % (253 of 360)	39.1 % (109 of 279)	95.2 % (20 of 21)
Suger, PO4	44.4 % (205 of 462)	65.1 % (164 of 252)	19.5 % (41 of 210)
Nucleobase	89.4 % (177 of 198)	82.4 % (89 of 108)	98.6 % (68 of 69)	95.2 % (20 of 21)
Aromatic	98.3 % (177 of 180)	98.9 % (89 of 90)	98.6 % (68 of 69)	95.2 % (20 of 21)

1. Loop E of 5S rRNA from spinach chloroplasts

GGGUGACGAU ACUGUAGGCG AGAGCCUGCG GAAAAAUAGC CC

Show sample condition

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

Release date

2003-10-15

Citation

The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts
Vallurupalli, P., Moore, P.B.
J. Mol. Biol. (2003), 325, 843-856, PubMed 12527295 , DOI: , Abstract

Related entities 1. Loop E of 5S rRNA from spinach chloroplasts, : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

1 1H-15NHSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

2 1H-13CHSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

3 1H-13CNOESY-HMQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

4 HCcH COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

5 Relay HCcH COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

6 Double Relay HCcH COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

7 IPAP HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

8 IPAP HCcH COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

9 IPAP Relay HCcH COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

10 NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

11 DQF-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian UnityPlus (500 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 K, pH 6.5

#	Name	Concentration
1	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
2	NaCl	50 mM
3	Cacodylate	2.5 mM
4	EDTA	0.1 mM
5	D2O	10 %
6	H2O	90 %

Sample #2

Temperature 298 K, pH 6.5

#	Name	Concentration
7	Loop E of 5S rRNA from spinach chloroplasts	1.5 mM
8	NaCl	50 mM
9	Cacodylate	2.5 mM
10	EDTA	0.1 mM
11	D2O	100 %

Sample #3

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
13	NaCl		50 mM
14	Cacodylate		2.5 mM
15	EDTA		0.1 mM
16	D2O		10 %
17	H2O		90 %

Sample #4

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	Loop E of 5S rRNA from spinach chloroplasts	[U->90% 13C; U->90% 15N]	1.5 mM
19	NaCl		50 mM
20	Cacodylate		2.5 mM
21	EDTA		0.1 mM
22	D2O		100 %

nullKeywords 5 S rRNA, L25, loop E, NMR, Spinacia oleracia

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Found 1 Document (1.424 seconds)

BMRB: 5919

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Related entities 1. Loop E of 5S rRNA from spinach chloroplasts, : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

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