BMRB: 5920

1H, 13C and 15N backbone and side-chain chemical shift assignments of PrrA effector domain from R. sphaeroides

Authors

Laguri, C., Phillips-jones, M.K., Williamson, M.P.

Assembly

PrrA C-terminal domain

Entity

1. PrrA C-terminal domain (polymer, Thiol state: not reported), 81 monomers, 9459.691 Da Detail

MGSSHHHHHH SSGLVPRGSH MLAKGESLPP PPENPMSADR VRWEHIQRIY EMCDRNVSET ARRLNMHRRT LQRILAKRSP R

Formula weight

9459.691 Da

Source organism

Cereibacter sphaeroides

Exptl. method

NMR

Refine. method

DISTANCE AND DIHEDRAL RESTRAINTS (TALOS)

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 95.1 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (902 of 948)	95.3 % (482 of 506)	94.5 % (342 of 362)	97.5 % (78 of 80)
Backbone	96.8 % (457 of 472)	97.5 % (155 of 159)	96.2 % (230 of 239)	97.3 % (72 of 74)
Sidechain	94.0 % (520 of 553)	94.2 % (327 of 347)	93.5 % (187 of 200)	100.0 % (6 of 6)
Aromatic	75.0 % (42 of 56)	75.0 % (21 of 28)	74.1 % (20 of 27)	100.0 % (1 of 1)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. PrrA

MGSSHHHHHH SSGLVPRGSH MLAKGESLPP PPENPMSADR VRWEHIQRIY EMCDRNVSET ARRLNMHRRT LQRILAKRSP R

Show sample condition

Sample

Temperature 275 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PrrA	[U-95% 13C; U-95% 15N]		1.0 mM
2	ammonium sulfate			200 mM
3	NaCl			50 mM
4	sodium phosphate			50 mM
5	DTT			10 mM

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LACS Plot; CA

Referencing offset: 0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 5 models in PDB: 1UMQ, Strand ID: A Detail

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Release date

2003-12-18

Citation

Solution structure and DNA binding of the effector domain from the global regulator PrrA (RegA) from Rhodobacter sphaeroides: insights into DNA binding specificity
Laguri, C., Phillips-jones, M.K., Williamson, M.P.
Nucleic Acids Res. (2003), 31, 6778-6787, PubMed 14627811 , DOI: , Abstract

Related entities 1. PrrA C-terminal domain, : 1 : 16 entities Detail