BMRB: 5923

Backbone 1H, 13C, and 15N Chemical Shift Assignments for T22G mutant of N-terminal SH3 domain of the Drosophila protein drk

Authors

Bezsonova, I.YU., Singer, A., Choy, W., Tollinger, M., Forman-Kay, J.D.

Assembly

T22G mutant of the N-terminal SH3 domain of the Drosophila protein drk

Entity

1. T22G mutant of the N-terminal SH3 domain of the Drosophila protein drk (polymer, Thiol state: not present), 59 monomers, 6672.362 Da Detail

MEAIAKHDFS ATADDELSFR KGGILKILNM GDDSNWYRAE LDGKEGLIPS NYIEMKNHD

Formula weight

6672.362 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 24.2 %, Completeness (bb): 45.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	24.2 % (164 of 679)	14.4 % (51 of 354)	23.7 % (62 of 262)	81.0 % (51 of 63)
Backbone	45.2 % (159 of 352)	41.8 % (51 of 122)	33.1 % (57 of 172)	87.9 % (51 of 58)
Sidechain	2.1 % (8 of 381)	0.0 % (0 of 232)	5.6 % (8 of 144)	0.0 % (0 of 5)
Aromatic	7.1 % (4 of 56)	0.0 % (0 of 28)	14.8 % (4 of 27)	0.0 % (0 of 1)
Methyl	0.0 % (0 of 52)	0.0 % (0 of 26)	0.0 % (0 of 26)

1. N-terminal SH3 domain of the Drosophila adapter protein drk

MEAIAKHDFS ATADDELSFR KGGILKILNM GDDSNWYRAE LDGKEGLIPS NYIEMKNHD

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Sample

Temperature 293 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal SH3 domain of the Drosophila adapter protein drk	[U-13C; U-15N]		1.0 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2A37, Strand ID: A Detail

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Release date

2006-01-11

Citation

Structural comparison of the unstable drkN SH3 domain and a stable mutant
Bezsonova, I.YU., Singer, A., Choy, W., Tollinger, M., Forman-Kay, J.D.
Biochemistry (2005), 44, 15550-15560, PubMed 16300404 , DOI 10.1021/bi0512795 , Abstract

Related entities 1. T22G mutant of the N-terminal SH3 domain of the Drosophila protein drk, : 1 : 5 : 389 entities Detail

Interaction partners 1. T22G mutant of the N-terminal SH3 domain of the Drosophila protein drk, : 13 interactors Detail

Experiments performed 3 experiments Detail