BMRB: 5926

Residual Dipolar Couplings of rubredoxin

Authors

Tian, F., Valafar, H., Prestegard, J.H.

Assembly

Rubredoxin

Entity

1. Rubredoxin (polymer, Thiol state: all other bound), 53 monomers, 5858.428 Da Detail

AKYVCKICGY IYDEDAGDPD NGVSPGTKFE EIPDDWVCPI CGAPKSEFEK LED

2. ZN (non-polymer), 65.409 Da

Total weight

5923.8374 Da

Max. entity weight

5858.428 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS8:SG	2:ZN1:ZN
3	na	sing	1:CYS38:SG	2:ZN1:ZN
4	na	sing	1:CYS41:SG	2:ZN1:ZN

Source organism

Pyrococcus furiosus

Exptl. method

NMR

Refine. method

RDC directed fragment assembly

Data set

RDCs

Protein Blocks Logo

Calculated from 1 models in PDB: 1RWD, Strand ID: A Detail

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RDC

419 RDC values in 2 lists
Field strength (¹H) 0.0 MHz Detail

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Experiment name 15N coupled HSQC, soft HNCA-E.COSY, modified HNCO

List #1 Phage_residual_dipolar_couplings, RDC code 1DHAH, Field strength (¹H) 0.0 MHz, Details The data were collected at 500 and 800 MHz. The error is the estimated experimental precision. Our computational methods require the errors to be expanded because the ability to fit the data to a model is more affected by assumptions about local geometry (i.e bond lengths, planarity, orientation, etc.)

List #2 Bicelle_residual_dipolar_couplings, RDC code 1DHACA, Field strength (¹H) 0.0 MHz, Details The data were collected at 500 and 800 MHz. The error is the estimated experimental precision. Our computational methods require the errors to be expanded because the ability to fit the data to a model is more affected by assumptions about local geometry (i.e bond lengths, planarity, orientation, etc.)

List #2 Bicelle_residual_dipolar_couplings, RDC code 1DHAH, Field strength (¹H) 0.0 MHz, Details The data were collected at 500 and 800 MHz. The error is the estimated experimental precision. Our computational methods require the errors to be expanded because the ability to fit the data to a model is more affected by assumptions about local geometry (i.e bond lengths, planarity, orientation, etc.)

List #2 Bicelle_residual_dipolar_couplings, RDC code 1DHC, Field strength (¹H) 0.0 MHz, Details The data were collected at 500 and 800 MHz. The error is the estimated experimental precision. Our computational methods require the errors to be expanded because the ability to fit the data to a model is more affected by assumptions about local geometry (i.e bond lengths, planarity, orientation, etc.)

List #2 Bicelle_residual_dipolar_couplings, RDC code 1DHN, Field strength (¹H) 0.0 MHz, Details The data were collected at 500 and 800 MHz. The error is the estimated experimental precision. Our computational methods require the errors to be expanded because the ability to fit the data to a model is more affected by assumptions about local geometry (i.e bond lengths, planarity, orientation, etc.)

Release date

2003-08-28

Citation

A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones
Tian, F., Valafar, H., Prestegard, J.H.
J. Am. Chem. Soc. (2001), 123, 11791-11796, PubMed 11716736 , DOI: , Abstract

Related entities 1. Rubredoxin, : 1 : 2 : 29 : 176 entities Detail

Experiments performed 3 experiments Detail